PAF1-complex-mediated histone methylation of FLOWERING LOCUS C chromatin required for the vernalization-responsive, winter-annual habit in Arabidopsis
PAF1-complex-mediated histone methylation of FLOWERING LOCUS C chromatin required for the vernalization-responsive, winter-annual habit in Arabidopsis
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DOI：
10.1101/gad.1244504
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发表时间：
2004-11-15
影响因子：
10.5
通讯作者：
Amasino, RM
中科院分区：
文献类型：
--
作者：
He, YH;Doyle, MR;Amasino, RM
拟南芥的冬季一年生习性（通常涉及需要暴露在冬季的寒冷中才能在春季开花）主要是由于相对高水平的FLC表达对开花的抑制。暴露于长时间的寒冷减弱FLC的表达，通过一个过程称为春化，从而允许开花发生在春天。在这里，我们表明，非春化的冬季一年生植物的FLC表达特性的升高需要两个基因，早开花7（ELF7）和早开花8（ELFS），这是同源的组件的PAF 1复合体的酿酒酵母。此外，ELF 7和ELF 8也是开花阻遏物的FLC进化枝中的其他基因如MAF 2和FLM的表达所必需的。FLC、FLM和MAF2参与多种开花途径，这些途径解释了elf7和elf8突变对开花行为的广泛影响。ELF 7和ELF 8是增强FLC染色质中组蛋白3在Lys 4处的三甲基化所必需的。FLC染色质的这种修饰似乎是将FLC表达提高到可以延迟未春化的植物开花的水平所必需的。ELF7，ELF8，和其他先前描述的基因在开花抑制剂的染色质的修饰中的作用的模型。
The winter-annual habit (which typically involves a requirement for exposure to the cold of winter to flower in the spring) in Arabidopsis thaliana is mainly due to the repression of flowering by relatively high levels of FLC expression. Exposure to prolonged cold attenuates FLC expression through a process known as vernalization and thus permits flowering to occur in the spring. Here we show that the elevated FLC expression characteristic of nonvernalized winter annuals requires two genes, EARLY FLOWERING 7 (ELF7) and EARLY FLOWERING 8 (ELFS), that are homologs of components of the PAF1 complex of Saccharomyces cerevisiae. Furthermore, ELF7 and ELF8 are also required for the expression of other genes in the FLC clade of flowering repressors such as MAF2 and FLM. FLC, FLM, and MAF2 are involved in multiple flowering pathways that account for the broad effects of elf7 and elf8 mutations on flowering behavior. ELF7 and ELF8 are required for the enhancement of histone 3 trimethylation at Lys 4 in FLC chromatin. This modification of FLC chromatin appears to be required to elevate FLC expression to levels that can delay flowering in plants that have not been vernalized. A model of the role of ELF7, ELF8, and other previously described genes in the modification of the chromatin of flowering repressors is presented.