Calcineurin mediates the immune response of hemocytes through NF-kappaB signaling pathway in pearl oyster (Pinctada fucata).
Calcineurin mediates the immune response of hemocytes through NF-kappaB signaling pathway in pearl oyster (Pinctada fucata).
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DOI：
10.1016/j.fsi.2009.11.001
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发表时间：
2010-02
影响因子：
4.7
通讯作者：
Changzhong Li;Jian Liang;Zhuojun Ma;Yilin Hu;Zhenguang Yan;Qing Li;Zi Fang;Hongzhong Wang;Guiyou Zhang;L. Xie;Rongqing Zhang
中科院分区：
文献类型：
--
作者：
Changzhong Li;Jian Liang;Zhuojun Ma;Yilin Hu;Zhenguang Yan;Qing Li;Zi Fang;Hongzhong Wang;Guiyou Zhang;L. Xie;Rongqing Zhang
钙调神经磷酸酶（Calcineurin，CN）是一种多功能蛋白，在哺乳动物体内通过多种信号通路介导免疫应答，但CN在软体动物血细胞免疫应答中的作用尚不清楚。本研究以合浦珠母贝为研究对象，检测了CN在不同组织中的分布以及Pf-CNA和Pf-CNB基因在合浦珠母贝血细胞中的表达。制备血细胞单层后，检测CN酶活性、IκBα降解水平、iNOS活性、NO和IL-2的产生对脂多糖（LPS）和环孢素A（CsA）刺激的反应。血细胞CN活性对LPS的刺激和CsA的抑制均十分敏感。最重要的是，LPS可诱导血细胞IκBα降解，CsA可减弱其降解。因此，iNOS的活性升高，NO的产生增加。此外，我们还发现LPS可促进IL-2的合成，而CsA则明显减弱其合成。体内细菌清除实验表明，CsA显著降低了珍珠蚌体内细菌清除能力。以上结果首次揭示了CN通过激活NF-κB信号通路介导贝类血细胞的免疫应答。
Calcineurin (CN), a multifunctional protein, mediates the immune response through diverse signaling pathways in mammals, while the function of CN in the immune response of molluscan hemocytes still remains unclear. In the present study, we detected the distribution of CN in various tissues and the expression levels of Pf-CNA and Pf-CNB gene in hemocytes of Pinctada fucata. After the preparation of hemocyte monolayers, we checked the response of enzymatic activity of CN, the degradation level of IκBα, the activity of iNOS and the production of NO, and IL-2 to the challenge of lipopolysaccharide (LPS) and cyclosporin A (CsA). CN activity in hemocytes was very sensitive to both the stimulation of LPS and the inhibition of CsA. Most importantly, IκBα degradation in hemocytes was induced by LPS and attenuated by CsA. Consequently, the activity of iNOS was elevated and the production of NO was increased. Additionally, we found that the synthesis of IL-2 was increased by LPS but was apparently weakened by CsA. In vivo bacterial clearance experiments showed that CsA significantly decreased the ability of in vivo bacteria clearance in pearl oyster. All the results revealed, for the first time, that CN mediated the immune response of molluscan hemocytes via activating NF-κB signaling pathway.