A land plant specific VPS13 mediates polarized vesicle trafficking in germinating pollen
A land plant specific VPS13 mediates polarized vesicle trafficking in germinating pollen
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DOI：
10.1101/2022.11.01.514778
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发表时间：
2022-11
期刊：
影响因子：
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通讯作者：
Surachat Tangpranomkorn;M. Igarashi;Fumiko Ishizuna;Y. Kato;Takamasa Suzuki;Sota Fujii;S. Takayama
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文献类型：
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作者：
Surachat Tangpranomkorn;M. Igarashi;Fumiko Ishizuna;Y. Kato;Takamasa Suzuki;Sota Fujii;S. Takayama
花粉具有从干燥状态转变为极其快速生长状态的非凡能力。在授粉过程中，花粉从接触雌蕊处接受水分和Ca ~（2+），这将是花粉管萌发的方向性线索。随后快速激活的定向泡运输必须支持花粉管生长，但导致这一过程的分子机制在很大程度上是未知的。在这里，我们表明，植物特有的VPS 13，AtVPS 13 a，介导的泡运输过程中的极化过程中在拟南芥花粉。AtVPS 13 a基因敲除严重影响花粉萌发和脂滴排出，而授粉后的Ca 2+动态没有变化。AtVPS 13 a和一个分泌囊泡标记的细胞分布模式是同步的，在极化花粉中的Ca 2+动态略有延迟。AtVPS 13 a的缺失导致花粉萌发过程中细胞壁沉积减少。这些结果表明，AtVPS 13 a介导花粉极化，通过调节适当的定向囊泡运输后Ca 2+信号。
Pollen has an extraordinary ability to convert from a dry state to an extremely rapidly growing state. During pollination, pollen receives water and Ca2+ from the contacting pistil, which will be a directional cue for pollen tube germination. The subsequent rapid activation of directional vesicular transport must support the pollen tube growth, but the molecular mechanism leading to this process is largely unknown. Here we show that a plant-specific VPS13, AtVPS13a, mediates vesicle trafficking during the polarization process in Arabidopsis pollen. AtVPS13a knockout severely affected pollen germination and lipid droplet discharge, while Ca2+-dynamics after pollination was unchanged. Cellular distribution patterns of AtVPS13a and a secretory vesicle marker were synchronized, with a slight delay to the Ca2+-dynamics in polarizing pollen. The absence of AtVPS13a led to reduced cell wall deposition during pollen germination. These results suggest that AtVPS13a mediates pollen polarization, by regulating proper directional vesicular transport following Ca2+-signaling.