CircRNA_101951 promotes migration and invasion of colorectal cancer cells by regulating the KIF3A-mediated EMT pathway
CircRNA_101951 promotes migration and invasion of colorectal cancer cells by regulating the KIF3A-mediated EMT pathway
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DOI：
10.3892/etm.2020.8600
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发表时间：
2020-05-01
影响因子：
2.7
通讯作者：
Cao, Ming-Zheng
中科院分区：
文献类型：
--
作者：
Li, Yue-Feng;Pei, Fu-Lai;Cao, Ming-Zheng
结直肠癌(CRC)是世界上最致命的肿瘤类型之一。环状RNA(CircRNAs)是RNA的共价闭合环，在多种肿瘤的增殖和转移中起着至关重要的作用。本研究对一种新的CircRNA--CircRNA_101951在结直肠癌中的表达、功能及其分子作用机制进行了研究。采用逆转录-定量聚合酶链式反应(RT-qPCR)检测CIRCCRNA_101951在大肠癌组织和细胞系中的表达。分别用四甲基偶氮唑盐比色法、集落形成实验、流式细胞仪和细胞迁移侵袭实验检测细胞增殖、克隆形成能力、细胞凋亡、细胞周期和细胞迁移侵袭能力。用RT-qPCR和Western印迹方法检测CircRNA_101951对KIF3A相关基因表达的影响。结果表明，在结直肠癌组织和细胞系中，CircRNA_101951的表达增加。CircRNA_101951的下调抑制了细胞的增殖和集落形成，抑制了细胞的迁移和侵袭。此外，通过检测KIF3A和间充质转化相关蛋白的表达水平，发现CircRNA_101951的下调阻断了KIF3A介导的上皮-间充质转化途径。综上所述，目前的数据显示CircRNA_101951可能作为结直肠癌患者的潜在生物标志物，并为抑制CircRNA_101951可能成为结直肠癌的一种潜在的治疗策略提供了新的见解。
Colorectal cancer (CRC) is one of the most lethal tumor types worldwide. Circular RNAs (circRNAs), which are covalent closed loops of RNA, perform vital roles for the proliferation and metastasis of a variety of tumor types. In the present study, the expression, function and molecular mechanisms of action of a novel circRNA, circRNA_101951, were examined in CRC. The expression levels of circRNA_101951 in CRC tissue and cell lines were examined using reverse transcription-quantitative (RT-qPCR). Cell proliferation, the clone formation ability, cell apoptosis, the cell cycle and the cell migratory and invasive abilities were examined using MTT assays, colony formation assays, flow cytometric assays, and cell migration and invasion assays, respectively. The effects of circRNA_101951 on Kinesin II family member 3A (KIF3A) related gene expression were examined using RT-qPCR and western blot assays. The results indicated that circRNA_101951 was increased in CRC tissues and cell lines. The downregulation of circRNA_101951 inhibited cell proliferation and colony formation as well as cell migration and invasion of CRC cell lines. In addition, the downregulation of circRNA_101951 blocked the KIF3A-mediated epithelial-mesenchymal transition (EMT) pathway, which was detected by examining the expression levels of KIF3A and EMT related proteins. In conclusion, the current data revealed that circRNA_101951 may act as a potential biomarker for patients with CRC, and provided a novel insight demonstrating that the suppression of circRNA_101951 may be a potential therapeutic strategy for CRC.