The Effect of 1α,25(OH)2D3 on Osteogenic Differentiation of Stem Cells from Dental Pulp of Exfoliated Deciduous Teeth
The Effect of 1α,25(OH)2D3 on Osteogenic Differentiation of Stem Cells from Dental Pulp of Exfoliated Deciduous Teeth
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发表时间：
2016-12
影响因子：
4.4
通讯作者：
F. Mojarad;I. Amiri;R. Rafatjou;Atousa Janeshin;M. Farhadian
中科院分区：
文献类型：
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作者：
F. Mojarad;I. Amiri;R. Rafatjou;Atousa Janeshin;M. Farhadian
问题陈述：人脱落乳牙干细胞是一群高度增殖的细胞，能够分化为成骨细胞、牙源性细胞、脂肪细胞和神经细胞。维生素D3代谢产物如1 α，25-二羟维生素D3是调节骨代谢的关键因素。目的：本研究旨在观察1 α，25-二羟维生素D3对乳牙脱落干细胞成骨分化的影响（碱性磷酸酶活性和茜素红染色）。材料和方法：从新鲜拔除的乳牙中取出牙髓，浸入消化液中。然后，将牙髓细胞浸入添加有10%胎牛血清的α-MEM（最低必需培养基）中。第三代后，从培养板中分离细胞并用于成骨分化。作为对照组，将细胞培养在成骨细胞培养基中。作为病例组，将细胞培养在添加100 nM 1 α，25（OH）2D3的成骨培养基中。第21天检测碱性磷酸酶（ALP）活性和茜素红染色，以评价成骨分化。结果采用t检验进行分析。结果：与对照组相比，1 α，25（OH）2D3处理后SHEDs的ALP活性显著升高（p = 0.002）。茜素红染色结果显示，1 α，25（OH）2D3诱导的细胞矿化结节明显增多（p <0.001）。结论：1 α，25（OH）2D3可促进SHEDs的成骨分化。结论：1 α，25（OH）2D3能促进成骨细胞的分化。
Statement of the Problem: Stem cells from human exfoliated deciduous teeth (SHEDs) are a population of highly proliferative cells, being capable of differentiating into osteogenic, odontogenic, adipocytes, and neural cells. Vitamin D3 metabolites such as 1α, 25-dihydroxyvitamin D3 are key factors in the regulation of bone metabolism. Purpose: The aim of this study was to investigate the effect of 1α, 25-dihydroxyvitamin D3 on osteogenic differentiation (alkaline phosphatase activity and alizarin red staining) of stem cells of exfoliated deciduous teeth. Materials and Method: Dental pulp was removed from freshly extracted primary teeth and immersed in a digestive solution. Then, the dental pulp cells were immersed in α-MEM (minimum essential medium) to which 10% fetal bovine serum was added. After the third passage, the cells were isolated from the culture plate and were used for osteogenic differentiation. As a control group, the cells were cultured in osteogenic cell culture medium. As the case group, the cells were cultured in osteogenic culture medium supplemented with 100 nM 1α,25 (OH)2D3. The alkaline phosphatase (ALP) activity and alizarin red staining were analyzed to evaluate the osteogenic differentiation at day 21. The results were analyzed by using t-test. Results: Compared with the control group, significant increase was observed in ALP activity of SHEDs after being treated with 1α,25(OH)2D3 (p= 0.002). Alizarin red staining demonstrated that the cells exposed to 1α,25(OH)2D3 induced higher mineralized nodules (p< 0.001). Conclusion: Osteoblast differentiation in SHEDs was stimulated by 1α,25(OH) 2D3. It can be concluded that 1α,25(OH)2D3 can improve osteoblastic differentiation.