High cell density cultivation of the lipid accumulating yeast Cryptococcus curvatus using glycerol as a carbon source
High cell density cultivation of the lipid accumulating yeast Cryptococcus curvatus using glycerol as a carbon source
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DOI：
10.1007/s002530050731
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发表时间：
1996-06-01
影响因子：
5
通讯作者：
Eggink, G
中科院分区：
文献类型：
--
作者：
Meesters, PAEP;Huijberts, GNM;Eggink, G
弯曲隐球菌是一种具有工业潜力的酵母，因为它可以在非常广泛的基质上生长和积累脂质。在这项研究中，我们描述了补料分批发酵模式下甘油的生长和脂质积累。我们进行了由两个阶段组成的发酵。第一阶段是生物质生产阶段，除了非常短时间的甘油耗尽外，没有营养限制。底物进料由溶解氧张力控制。在第二阶段引入氮限制，这导致脂质积累。这样，在 50 小时的发酵中即可达到 118 克/升的非常高的细胞密度。当脂质产生率为0.59 g脂质I-1 h(-1)时，细胞脂质含量为25%。生长和脂质积累阶段的特征是不同的细胞脂肪酸组成。在生长期，存在相对大量的 C18:2（亚油酸），它是膜脂的主要成分。 C18:0（硬脂酸）和 C18:1（油酸）是积累的甘油三酯的主要成分，因此 C18:2 的相对量在脂质积累阶段减少。
Cryptococcus curvatus is a yeast with industrial potential because it can grow and accumulate lipid on a very broad range of substrates. In this study we describe growth and lipid accumulation on glycerol in a fed-batch fermentation mode. We performed a fermentation consisting of two phases. The first phase is the biomass production phase in which there is no nutrient limitation except for very short periods of glycerol exhaustion. The substrate feed was controlled by the dissolved oxygen tension. In the second phase nitrogen limitation was introduced, which causes lipid accumulation. This way very high cell densities of 118 g/l in a 50-h fermentation could be reached. With a lipid production rate of 0.59 g lipid I-1 h(-1), a cellular lipid content of 25% was obtained. The growth and lipid accumulation phase are characterized by different cellular fatty acid compositions. In the growth phase, a relatively high amount of C18:2 (linoleic acid) is present, which is a major component of membrane lipids. C18:0 (stearic acid) and C18:1 (oleic acid) are major constituents of the accumulated triglycerides and therefore the relative amount of C18:2 decreases during the lipid accumulation phase.