Epithelial-Mesenchymal Transition in Chronic Rhinosinusitis: Differences Revealed Between Epithelial Cells from Nasal Polyps and Inferior Turbinates
Epithelial-Mesenchymal Transition in Chronic Rhinosinusitis: Differences Revealed Between Epithelial Cells from Nasal Polyps and Inferior Turbinates
复制标题
DOI：
10.1007/s00005-016-0409-7
复制
发表时间：
2017-04-01
影响因子：
3.2
通讯作者：
Wollenberg, Barbara
中科院分区：
文献类型：
--
作者：
Koennecke, Michael;Burmeister, Maike;Wollenberg, Barbara
慢性鼻-鼻窦炎(CRS)的发病机制至今仍不清楚。鼻息肉的组织重塑可能是炎症介质的结果，可能涉及上皮-间充质转化(EMT)和与EMT相关的特征，如鼻腔上皮细胞(NECS)中的细胞运动。我们确定CRS鼻息肉中的NEC在体内是否已经表现出EMT的特征，或者在体外对EMT的生长因子刺激有反应。鼻息肉组织同时表达上皮性和间叶性标记物。来自下鼻甲和鼻息肉的原代NEC对EMT诱导剂转化生长因子-β1和表皮生长因子(EGF)有不同的EMT标志物表达模式(E-钙粘素、N-钙粘素、蜗牛、鼻涕、扭曲)，但只有鼻息肉的NEC对转化生长因子-β1和EGF依赖的细胞迁移敏感。我们的数据表明，部分EMT与CRS患者鼻息肉的发病机制有关。此外，我们首次发现，鼻息肉和下鼻甲的上皮细胞在体外暴露于转化生长因子-β1或表皮生长因子后能够经历类似EMT的过程，但只有鼻息肉的NEC具有增强的细胞运动性。我们的数据提示CRS患者的NEC经历了部分EMT，这一过程可能参与了CRS的发病机制。
The pathogenesis of chronic rhinosinusitis (CRS) remains unclear to date. The tissue remodeling in nasal polyps may be the result of inflammatory mediators and may involve epithelial-mesenchymal transition (EMT) and EMT-associated features such as cell motility in nasal epithelial cells (NECs). We determined whether NEC in nasal polyps of CRS already display features of EMT in vivo or respond with EMT to growth factor stimulation in vitro. Nasal polyp tissues expressed both epithelial and mesenchymal markers. Primary NEC from inferior turbinates and nasal polyps responded to the EMT-inducing agents transforming growth factor (TGF)-beta 1 and epidermal growth factor (EGF) with different expression patterns of EMT markers (E-cadherin, N-cadherin, Snail, Slug, Twist), however, only NEC from nasal polyps were susceptible to TGF-beta 1 and EGF-dependent cell migration. Our data suggest that a partial EMT is associated with the pathogenesis of nasal polyps in CRS patients. Furthermore, we show for the first time that epithelial cells from both nasal polyps and inferior turbinates were able to undergo an EMT-like process following exposure to TGF-beta 1 or EGF in vitro but that only NEC from nasal polyps responded with enhanced cell motility. Our data suggest that NEC from CRS patients have undergo partial EMT and that this process may be involved in the pathogenesis of CRS.