Leishmania (Leishmania) chagasi is not vertically transmitted in dogs
Leishmania (Leishmania) chagasi is not vertically transmitted in dogs
复制标题
DOI：
10.1016/s0304-4017(01)00552-0
复制
发表时间：
2002-01-03
影响因子：
2.6
通讯作者：
Genaro, O
中科院分区：
文献类型：
--
作者：
Andrade, HM;de Toledo, VDCP;Genaro, O
人类或犬内脏利什曼病（CVL）传播的最常见和最重要的模式是通过受感染的白蛉叮咬。本研究探讨了自然感染的狗的后代中的恰加斯利什曼原虫（利什曼原虫）的垂直传播。因此，使用了18只患有CVL的雌性犬的63只幼犬。通过淋巴器官的寄生虫病理学和组织病理学检查以及成人（乳汁、子宫、胎盘、脾脏、肝脏和骨髓）和后代（脾脏、肝脏、淋巴结和骨髓）样本的聚合酶链反应（PCR）评价寄生虫存在情况。使用显微镜作为骨髓、脾和肝样品的金标准，计算PCR灵敏度和特异性。所有器官的特异性为100%，灵敏度为骨髓100%，脾脏71.4%，肝脏66.6%。对63只子代进行了骨髓涂片（n = 63）、脾脏（n = 25）、肝脏（n = 12 = 25）和淋巴结（n = 25）的组织病理学和印片检查，以评估先天性传播。对从56个后代中收集的92个样品进行PCR。对后代进行的测试均为阴性。尽管血清阳性成人胎盘PCR阳性，但无法确认CVL的垂直传播（观察到的患病率的95%置信区间）。(C)2002 Elsevier Science B.V.保留所有权利。
The most frequent and most important mode of human or canine visceral leishmaniasis (CVL) transmission is through the bite of infected sand flies. This study investigates Leishmania (Leishmania) chagasi vertical transmission in offspring of naturally infected dogs. Thus 63 puppies from 18 female dogs with CVL were used. Parasite presence was evaluated through parasitologic and histopathologic examination of lymphatic organs, as well as polymerase chain reaction (PCR) on samples from adults (milk, uterus, placenta, spleen, liver and bone marrow) and offspring (spleen, liver, lymph nodes and bone marrow). PCR sensitivity and specificity were calculated using a microscope as the gold standard on samples of bone marrow, spleen and liver. Specificity was 100% for all organs and sensitivity was 100% for bone marrow, 71.4% for spleen and 66.6% for liver. Bone marrow smears (n = 63), histopathology and imprint of spleen (n = 25), liver (12 = 25) and lymph nodes (n = 25) were performed to evaluate congenital transmission in the 63 offspring. PCR was done on 92 samples collected from 56 of the offspring. No test performed on the offspring was positive. It was not possible to confirm vertical transmission of CVL (95% confidence interval for the observed prevalence), despite positive PCR in the placenta of seropositive adults. (C) 2002 Elsevier Science B.V. All rights reserved.