Promotion of Metabolite Synthesis in Isaria cicadae, a Dominant Species in the Cicada Flower Microbiota, by Cicada Pupae
Promotion of Metabolite Synthesis in Isaria cicadae, a Dominant Species in the Cicada Flower Microbiota, by Cicada Pupae
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DOI：
10.1021/acs.jafc.9b02705
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发表时间：
2019-08-07
影响因子：
6.1
通讯作者：
Shi, Jin-Song
中科院分区：
文献类型：
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作者：
Chunyu, Yan-Jie;Lu, Zhen-Ming;Shi, Jin-Song
蝉花，是食用菌和药用蘑菇，是寄生在蝉蛹幼虫身上的真菌蝉花的子实体。我们假设宿主因子可能对蝉的代谢物合成具有刺激活性。在这里，我们首先通过高通量测序比较了不同地理区域、隔室和生长阶段的不同野生蝉花的微生物群落结构。 Isaria cicadae TZC-3 是所有真菌群落中最丰富的操作分类单位 (OTU6782) 的分离物，是从野生蝉花中分离出来的。此外，在深层培养中研究了蝉蛹对蝉Isaria cicadae TZC-3代谢物合成的影响。蝉蛹粉(4%)培养的菌丝体胞间多糖、腺苷、N6-(2-羟乙基)-腺苷、游离氨基酸、水解单糖含量较对照组显着升高。这表明蝉蛹可以作为蝉蛹代谢物合成的诱导子。
Cicada flowers, which are edible and medicinal mushrooms, are the fruiting bodies of Isaria cicadae, a fungus that is parasitic on the larvae of cicada pupae. We hypothesize that host factors might possess stimulatory activity on metabolite synthesis in Isaria cicadae. Here, we first compared the microbial community structures of different wild cicada flowers across geographical regions, compartments, and growth stages via high-throughput sequencing. Isaria cicadae TZC-3, an isolate of the most abundant operational taxonomic unit (OTU6782) in all the fungal communities, was isolated from wild cicada flowers. Furthermore, the effects of cicada pupae on metabolite synthesis in Isaria cicadae TZC-3 were studied in submerged culture. The contents of intercellular polysaccharides, adenosine, N6-(2-hydroxyethyl)-adenosine, free amino acids, and hydrolyzed monosaccharides in the mycelia cultured with cicada pupa powder (4%) were significantly increased as compared with the contents in the control group. This indicates that a cicada pupa can act as an elicitor for metabolite synthesis in Isaria cicadae.