Special features of the priming process for a secretory IgA response. B cell priming with cholera toxin.
Special features of the priming process for a secretory IgA response. B cell priming with cholera toxin.
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DOI：
10.1084/jem.153.3.534
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发表时间：
1981-03-01
期刊：
影响因子：
--
通讯作者：
Cebra JJ
中科院分区：
文献类型：
--
作者：
Fuhrman JA;Cebra JJ
通过十二指肠内或胃肠外途径给予霍乱毒素/类毒素可使派尔集合淋巴结（PP）和远端淋巴组织中抗原敏感性B细胞的频率增加50倍以上。用毒素进行粘膜引发的特殊性在于其在产生次级B细胞方面的独特有效性，次级B细胞的后代仅表达伊加，并且这种细胞在PP中出现的频率最高，在脾脏中出现的数量可观。因此，这种故意的十二指肠内免疫似乎模仿了肠道细菌定植诱导的自然引发过程，我们假设这是常规饲养小鼠PP中细菌决定簇特异性IgA定型细胞频率高的原因。此外，作为十二指肠内免疫的结果，抗原特异性记忆B细胞散布到远离抗原应用的部位，包括与呼吸道粘膜相关的淋巴滤泡。因此，PP中细胞的直接抗原刺激通过抗原敏感性次级B细胞的分裂、分化和去血导致粘膜和全身部位之间的有效交叉致敏。
Administration of cholera toxin/toxoid by either intraduodenal or parenteral routes increases the frequency of antigen-sensitive B cells in Peyer's patches (PP) and in distant lymphoid tissues greater than 50- fold. The special feature of mucosal priming with toxin is its unique effectiveness at generating secondary B cells, whose progeny express IgA exclusively, and such cells appear in highest frequency in PP and in appreciable numbers in spleen. Thus, this deliberate intraduodenal immunization seems to mimic the natural priming process induced by enteric bacterial colonization, which we have postulated to account for the high frequencies of IgA-committed cells specific for bacterial determinants in the PP of conventionally reared mice. furthermore, as a result of intraduodenal immunization, antigen-specific memory B cells are disseminated to sites distant form that of antigen application, including the lymphoid follicles associated with the respiratory mucosa. Direct antigenic stimulation of cells in the PP therefore results in effective cross-priming among mucosal and systemic sites through division, differentiation, and disemination of antigen- sensitive secondary B cells.