Effects of a dental adhesive incorporating antibacterial monomer on the growth, adherence and membrane integrity of Streptococcus mutans
Effects of a dental adhesive incorporating antibacterial monomer on the growth, adherence and membrane integrity of Streptococcus mutans
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含有抗菌单体的牙科粘合剂对变形链球菌生长、粘附和膜完整性的影响
DOI：
10.1016/j.jdent.2008.12.004
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发表时间：
2009-04-01
影响因子：
4.4
通讯作者：
Ma, Sai
中科院分区：
文献类型：
--
作者：
Li, Fang;Chen, Jihua;Ma, Sai
目的：本研究试图将抗菌单体甲基丙烯氧基乙基十六烷基二甲基氯化铵（DMAE-CB）加入到商用牙胶粘剂中，并评价DMAE-CB加入的牙胶粘剂对变形链球菌的抗菌活性。方法：将DMAE-CB以3% （w/v）掺入临床使用的牙科胶粘剂Single Bond 2中。不含DMAE-CB的单键2为阴性对照；Clearfil Protect Bond含有一种深入研究的抗菌单体MDPB，作为阳性对照。通过膜接触试验和吸光度测定，研究了固化胶粘剂及其洗脱液对变形链球菌生长的影响。采用共聚焦激光扫描显微镜（CLSM）结合荧光指示剂研究了固化胶粘剂对变形链球菌（S. mutans）粘附性和膜完整性的影响。结果：与阴性对照比较，dmae - cb -固化牙胶粘剂和阳性对照对变形链球菌的生长有抑制作用（P < 0.05），而其洗脱液的抑菌活性不明显。CLSM图像的荧光分析表明，固化后的dmae - cb -结合的黏合剂和阳性对照可以抑制变形链球菌的粘附，并对细菌膜的完整性产生不利影响（P < 0.05）。结论：DMAE-CB的掺入可通过影响变形链球菌的生长、粘附和膜完整性，使聚合后的牙胶粘剂具有接触抗菌活性。(C) 2008 Elsevier Ltd版权所有。
Objectives: This study was attempted to incorporate an antibacterial monomer, methacryloxylethyl cetyl dimethyl ammonium chloride (DMAE-CB), into a commercial dental adhesive and to evaluate the antibacterial activity of the DMAE-CB-incorporated adhesive after being cured against Streptococcus mutans.Methods: DMAE-CB was incorporated at 3% (w/v) into a clinically used dental adhesive, Single Bond 2. Single Bond 2 without DMAE-CB was served as a negative control; Clearfil Protect Bond, containing an intensively researched antibacterial monomer MDPB, was enrolled as a positive control. The effects of the cured adhesives and their eluents on the growth of S. mutans were determined by film contact test and absorbance measurement, respectively. The effects of the cured adhesives on the adherence and membrane integrity of S. mutans were investigated using confocal laser scanning microscopy (CLSM) in conjunction with fluorescent indicators.Results: Compared with negative control, the cured DMAE-CB-incorporated dental adhesive and positive control were found to exhibit inhibitory effect on the growth of S. mutans (P < 0.05), whereas their eluents did not show detectable antibacterial activity. Moreover, the fluorescence analysis of CLSM images demonstrated that the cured DMAE-CB-incorporated adhesive and positive control could hamper the adherence of S. mutans and exert detrimental effect on bacterial membrane integrity (P < 0.05).Conclusions: The incorporation of DMAE-CB can render dental adhesive with contact antibacterial activity after polymerization via influencing the growth, adherence and membrane integrity of S. mutans. (C) 2008 Elsevier Ltd. All rights reserved.