Molecular phylogeny of the Pertusariaceae supports secondary chemistry as an important systematic character set in lichen-forming ascomycetes.
Molecular phylogeny of the Pertusariaceae supports secondary chemistry as an important systematic character set in lichen-forming ascomycetes.
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DOI：
10.1016/j.ympev.2004.04.014
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发表时间：
2004-10
影响因子：
4.1
通讯作者：
I. Schmitt;H. Lumbsch
中科院分区：
文献类型：
--
作者：
I. Schmitt;H. Lumbsch
Pertusariaceae是一种多样的，世界性的壳状地衣形成真菌。它特别富含次级代谢产物，含有来自不同物质类别的各种化合物，如氧杂蒽酮、缩酚酮、缩酚酮和缩酚酮。基于形态学和化学的研究提供了相互矛盾的观点分类群之间的关系，属间的系统发育关系在百日咳科仍然是不确定的。为了评估这些关系，我们产生了基于核大亚基（nu LSU）和线粒体小亚基（mt SSU）rRNA基因的核苷酸序列的同源性。对于分类群的一个子集，我们还测序了线粒体大亚基（mt LSU）rDNA。我们研究了总共49个分类群，从家庭百日咳科和神秘的属Loxosporopsis，包括四个Agyrialean分类群用作外群。使用具有马尔可夫链蒙特卡罗树抽样（B/MCMC）的贝叶斯方法和最大简约法分析比对。在由此产生的系统发育估计中，百日咳属在其目前的范围内是多系的，包括三个主要分支：组、水痘组、水痘组。Loxosporopsis是密切相关的Pertusaria S.str.我们重新分析了形态，解剖和化学特征的分子研究的光，发现新的字符组合来描述单系实体。证实了鸡骨菌科特有次生化学成分的分类学意义。
The Pertusariaceae is a diverse, cosmopolitan group of crustose lichen-forming fungi. It is particularly rich in secondary metabolites, containing a variety of compounds from different substance classes, such as xanthones, depsides, depsidones, and depsones. Morphology and chemistry-based studies have provided conflicting views of relationships among taxa, and phylogenetic relationships among genera in the Pertusariaceae are still unsettled. To evaluate these relationships, we generated a phylogeny based on nucleotide sequences of the nuclear large subunit (nu LSU) and the mitochondrial small subunit (mt SSU) rRNA genes. For a subset of taxa, we additionally sequenced the mitochondrial large subunit (mt LSU) rDNA. We studied a total of 49 taxa from the family Pertusariaceae and the enigmatic genus Loxosporopsis including four Agyrialean taxa used as outgroups. The alignments were analyzed using a Bayesian approach with Markov Chain Monte Carlo tree sampling (B/MCMC), and maximum parsimony methods. In the resulting phylogenetic estimates the genus Pertusaria in its current circumscription is polyphyletic comprising three major clades: the Pertusaria s.str.-group, the Variolaria-group and the Varicellaria-group. Loxosporopsis is closely related to Pertusaria s.str. We re-analyzed morphological, anatomical, and chemical features in the light of the molecular study and found novel character combinations to describe monophyletic entities. The taxonomic significance of particular secondary chemical constituents in the Pertusariaceae is corroborated.