A Novel Layer-by-Layer Self-Assembly Biomimetic Hydroxyapatite-Cell Sheet for Bone Tissue Engineering
A Novel Layer-by-Layer Self-Assembly Biomimetic Hydroxyapatite-Cell Sheet for Bone Tissue Engineering
复制标题
用于骨组织工程的新型层层自组装仿生羟基磷灰石细胞片
DOI：
10.1166/jbt.2016.1526
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发表时间：
2016-12
影响因子：
0.1
通讯作者：
Qiu Xiaozhong
中科院分区：
文献类型：
--
作者：
Hu Tiantian;Xu Huiyong;Sang Lili;Wang Leyu;Dokiparty Krupa Daniel;Lv Hengyong;Ouyang Jun;Qiu Xiaozhong
基于羟基磷灰石微粒(MHAP)与MC3T3-E1成骨细胞之间的相互作用，研制了一种用于骨组织工程的新型生物逐层细胞片。LBL薄膜模仿了无机和有机基质的自然组合。通过活/死实验、苏木精-伊红(HE)染色和扫描电子显微镜(SEM)观察，对细胞片的性质进行表征。所得到的细胞片表现出良好的细胞活性，细胞外基质和MHAP颗粒完整。细胞膜与水凝胶的界面显示，深层活细胞与浅层细胞具有相同的形态结构。细胞片可以通过在细胞和培养皿中间沉积MHAP而与细胞培养皿分离。此外，该细胞板保持了较高的成骨能力，可以作为一种更理想的生物系统用于修复受损的骨组织，而不需要PIPAAM或任何其他辅助设备。
In this paper, a new biological layer-by-layer (LBL) cell sheet was developed for bone tissue engineering based on the interaction between hydroxyapatite micro-particles (mHAP) and MC3T3-E1 osteoblast cells. The LBL film mimicked a natural combination of inorganic and organic matrix. The properties of the cell sheets were characterized by live/dead assay, hematoxylin-eosin (HE) staining and scanning electron microscope (SEM) observation. The resulting cell sheet had exhibited well cell viability with the integrity of the extracellular matrix and mHAP particles. The interface between cell sheet and hydrogel showed that the living cells in deep layer have the same morphological structure as the cells on the superficial layer. The cell sheet could apart from the cell culture dish through deposited mHAP across in the middle of cells and the dish. In addition, the cell sheet maintained a high osteogenic capacity and could be taken as a more ideal bio-system for the repair of damaged bones without PIPAAm or any other ancillary equipment.