Patterns of integrin expression in a human mandibular explant model of osteoblast differentiation
Patterns of integrin expression in a human mandibular explant model of osteoblast differentiation
复制标题
DOI：
10.1016/s0003-9969(00)00114-x
复制
发表时间：
2001-03-01
影响因子：
3
通讯作者：
Walsh, S
中科院分区：
文献类型：
--
作者：
Bennett, JH;Carter, DH;Walsh, S
细胞-基质相互作用在调节成骨细胞分化和功能中至关重要。这些相互作用本身至少部分由整合素调节。虽然有一些来自哺乳动物模型的数据，但很少有研究比较成骨细胞谱系不同阶段的整合素表达。在这里，原代人下颌骨成骨细胞培养物在表皮生长因子（EGF）的存在下生长，给予增殖，分化程度较低的表型，或维生素D-3和氢化可的松（D + He），给予分化程度较高的表型。这些文化进行了比较，在EGF或D + He的情况下，通过荧光激活的细胞分选仪使用的面板的单克隆抗体，以特定的整合素异源二聚体制备的细胞。为了提供体内相关性，使用同一组抗体对新鲜冷冻的人下颌骨未矿化切片进行染色。在基线条件下，细胞强烈表达α（3）、α（5）、α（v）α（v）β（3）、β（3）和β（1）整联蛋白亚基，α（1）、α（2）和α（4）亚基表达水平较低。在EGF的存在下，表达增加。在D + He的情况下，α（3）和α（5）表达升高。免疫组织化学分析表明，成骨细胞谱系细胞中存在α（2）、α（3）、α（5）、α（v）、β（3）、β（1）和β（3）亚基; α（2）染色仅限于靠近骨表面的细胞，而α（v）、β（3）和β（3）最常位于骨细胞中。结果进一步证明成骨细胞谱系的连续阶段的细胞显示不同的整合素表达模式。这些整合素可能是重要的细胞基质相互作用，导致成骨细胞分化。(C)2001爱思唯尔科技有限公司版权所有。
Cell-matrix interaction is crucial in regulating osteoblast differentiation and function. These interactions are themselves regulated, at least in part, by integrins. Although there are some data from mammalian models, few studies have compared integrin expression at different stages of the osteoblast lineage. Here, primary human mandibular osteoblast cultures were grown in the presence of epidermal growth factor (EGF), giving a proliferative, less differentiated phenotype, or of vitamin D-3 and hydrocortisone (D + He), giving a more differentiated phenotype. These cultures were compared with those of cells prepared in the absence of EGF or D + He by fluorescence-activated cell sorter using a panel of monoclonal antibodies to specific integrin heterodimers. To provide in vivo correlation, the same panel of antibodies was used to stain fresh-frozen, undemineralised sections of human mandibular bone. Under baseline conditions the alpha (3), alpha (5), alpha (v) alpha (v)beta (3), beta (3) and beta (1) integrin subunits were expressed strongly by the cells, with low-level expression of the alpha (1), alpha (2) and alpha (4) subunits. In the presence of EGF there was increased a, expression. With D + He, alpha (3) and alpha (5) expression was elevated. Immunohistochemical analysis demonstrated alpha (2), alpha (3), alpha (5), alpha (v)beta (3), beta (1) and beta (3) subunits in cells of the osteoblast lineage; alpha (2) staining was restricted to cells close to the bone surface whilst alpha (v)beta (3) and beta (3) were most frequently localised in the osteocytes. The results proc ide evidence that cells at successive stages of the osteoblast lineage show different patterns of integrin expression. These integrins may be important in cell-matrix interactions leading to osteoblast differentiation. (C) 2001 Elsevier Science Ltd. All rights reserved.