Age-Related Changes in Bone Structure and Strength in Female and Male BALB/c Mice
Age-Related Changes in Bone Structure and Strength in Female and Male BALB/c Mice
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DOI：
10.1007/s00223-010-9359-y
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发表时间：
2010-06-01
影响因子：
4.2
通讯作者：
Silva, Matthew J.
中科院分区：
文献类型：
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作者：
Willinghamm, Mark D.;Brodt, Michael D.;Silva, Matthew J.
小鼠可能对骨骼老化的研究有用，但在其一生中骨骼结构和强度的变化方面的数据有限。我们获得了2、4、7、12和20个月大的雌性和雄性BALB/c小鼠的骨骼，并评估了它们的结构、密度和力学性能。股骨和桡骨中段微ct显示，在骨骼生长期间（2-7个月），骨横截尺寸（面积、转动惯量）迅速增大；在衰老过程中（7 ~ 20个月），脑皮层面积保持不变，惯性矩持续增加。女性的骨骼在年轻时比男性小，但在老年时则不是这样。全骨刚度和强度的变化反映了骨大小的变化，从2个月到7个月迅速增加，随后几乎没有变化。相反，断裂能量随老化而下降。皮层组织矿物质密度在生长过程中增加，并随年龄增长而保持不变。骨小梁的显微ct显示年龄相关的变化是位点依赖的。胫骨近端骨小梁BV/TV明显与年龄相关，男女均在4个月后渐进式下降；腰椎L5与年龄相关的衰退更为温和；尾椎Ca7 BV/TV随年龄增长而增加。总的来说，我们没有发现女性比男性有更明显的年龄相关恶化的证据。我们得出结论，衰老的雌性和雄性BALB/c小鼠的骨骼表现出许多在人类中看到的变化，因此是骨骼衰老研究的临床相关模型。
Mice may be useful for studies of skeletal aging, but there are limited data on changes in bone structure and strength over their life span. We obtained bones from female and male BALB/c mice at ages 2, 4, 7, 12, and 20 months and evaluated their structural, densitometric, and mechanical properties. MicroCT of the mid-diaphysis of the femur and radius indicated that during skeletal growth (2-7 months) bone cross-sectional size (area, moment of inertia) increased rapidly; during aging (7-20 months) cortical area was maintained, while moment of inertia continued to increase. Bones from females were smaller than those from males at young ages but not at later ages. Changes in whole-bone stiffness and strength reflected the changes in bone size, with a rapid increase from 2 to 7 months, followed by little or no change. In contrast, energy-to-fracture declined with aging. Cortical tissue mineral density increased during growth and was maintained with aging. MicroCT of trabecular bone revealed age-related changes that were site-dependent. The proximal tibia showed a clear pattern of age-related decline in trabecular BV/TV, with progressive decreases after 4 months in both sexes; lumbar vertebra L5 had more modest age-related declines; in contrast, caudal vertebra Ca7 had increasing BV/TV with aging. Overall, we found no evidence that females had more pronounced age-related deterioration than males. We conclude that bones from aging female and male BALB/c mice exhibit many of the changes seen in humans and are therefore a clinically relevant model for studies of skeletal aging.