Diversification within embryonic chick somites: differential response to notochord.
Diversification within embryonic chick somites: differential response to notochord.
复制标题
胚胎鸡体节内的多样化：对脊索的差异反应。
DOI：
10.1016/0012-1606(81)90292-x
复制
发表时间：
1981
影响因子：
2.7
通讯作者：
Lash,JW
中科院分区：
文献类型：
--
作者：
Cheney,CM;Lash,JW
脊椎动物胚胎的脊索和体节之间的组织相互作用建立了脊椎软骨的正确形状和构成。体节形成后不久，体节分化为软骨形成部分，即硬节，以及肌肉和皮肤形成部分，即皮肌节。从3 ~ 12日龄鸡胚（18 ~ 12 ~ 19期）上切下体节的这些组成部分，并在有或无脊索的情况下进行体外培养。结果发现，硬节细胞对脊索的反应是透明软骨结节的发生率增加，硫酸化糖胺聚糖的积累增加，蛋白聚糖的合成更大的聚集体，DNA积累增加，并加速DNA合成。皮肌刀未显示这些变化。这些结果表明，脊索促进软骨分化的硬骨节。在这些条件下，脊索在皮肌刀中不引起软骨形成。
The tissue interaction between the notochord and the somites of the vertebrate embryo establishes the proper shape and constitution of the vertebral cartilage. Soon after somite formation, the somite differentiates into a cartilage-forming part, the sclerotome, and a muscle and skin-forming part, the dermamyotome. These components of the somite were dissected from 3 1 2-day-old chick embryos (stage 18 1 2–19 and cultured in vitro in the presence or absence of notochord. It was found that the sclerotome cells respond to the notochord by an increased incidence of hyaline cartilage nodules, greater accumulation of sulfated glycosaminoglycans, synthesis of larger aggregates of proteoglycans, increased DNA accumulation, and accelerated DNA synthesis. The dermamyotome did not show these changes. These results indicate that the notochord enhances cartilage differentiation in the sclerotome. Under these conditions, the notochord did not elicit cartilage formation in the dermamyotome.