Serologic Evidence of Prevalent Avian H3 Subtype Influenza Virus Infection in Chickens
Serologic Evidence of Prevalent Avian H3 Subtype Influenza Virus Infection in Chickens
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DOI：
10.1637/8410-071708-reg.1
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发表时间：
2009-06-01
期刊：
影响因子：
1.4
通讯作者：
Liu, Jin-hua
中科院分区：
文献类型：
--
作者：
Pu, Juan;Zhang, Guo-zhong;Liu, Jin-hua
已知H3亚型流感病毒可感染禽类和哺乳动物，包括人类。然而，关于H3型流感病毒在中国鸡群中的流行情况，人们知之甚少。因此，对中国地区的鸡进行了血清学调查，以了解禽流感病毒H3亚型的血清阳性率。用血凝抑制试验(HI)和确证病毒中和试验(VN)对2006年7月至2007年6月从16个省和2个直辖市18个地区的173个鸡群采集的4598份血清标本进行了抗H3抗体检测。血清流行病学结果显示，在中国的部分地区，包括18个试验区中的12个地区，H3亚型禽流感病毒在鸡中流行，总体平均流行率为2.83%。173个鸡群中有44个样本HI/VN阳性，其中15个鸡群的HI/VN水平在10.00%~41.94%之间。在较老的鸡群和在较凉爽的季节采样的鸡群中观察到显著较高的血清阳性率。标准化比较显示，分别位于中国以南和东部的广东和江苏的H3血清阳性水平明显较高。这些结果第一次证明了在中国多个地点的鸡群中存在H3禽流感病毒感染的血清学证据。这些观察结果突出表明，需要在中国和其他地区继续对H3亚型和其他低致病性禽流感病毒进行流行病学监测。
H3-subtype influenza viruses are known to infect avian and mammalian species, including humans. However, little is known about the prevalence of H3 influenza virus infection in chicken populations in China. Therefore, a serologic survey of chickens was conducted in China to investigate the seroprevalence of avian H3-subtype influenza virus. Anti-H3 antibodies were assayed by using hemagglutination inhibition (HI) and confirmatory virus neutralization (VN) testing of 4598 serum samples, collected between July 2006 and June 2007, from 173 chicken flocks located in 18 areas that included 16 provinces and two municipalities. Seroepidemiologic results indicated that avian H3-subtype viruses were circulating in chickens in some regions of China, regions that included 12 of the 18 test areas, with an overall average prevalence rate of 2.83%. Samples from 44 of 173 flocks were HI/VN seropositive, including 15 flocks with levels that ranged from 10.00% to 41.94%. Significantly higher seroprevalence rates were observed in older chicken flocks and in those sampled in the cooler seasons. Standardized comparisons showed that Guangdong and Jiangsu, located in the south and east of China, respectively, had significantly higher levels of H3 seropositivity. For the first time, these results demonstrated serologic evidence for H3 avian influenza virus infection in chicken populations in several locations throughout China. These observations highlight the need for continued epidemiologic surveillance of the H3 subtype and for other low-pathogenic avian influenza viruses in China and other regions.