Porcine urinary bladder matrix-polypropylene mesh: a novel scaffold material reduces immunorejection in rat pelvic surgery
Porcine urinary bladder matrix-polypropylene mesh: a novel scaffold material reduces immunorejection in rat pelvic surgery
复制标题
猪膀胱基质-聚丙烯网：一种新型支架材料可减少大鼠盆腔手术中的免疫排斥
DOI：
10.1007/s00192-012-1745-8
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发表时间：
2012-09-01
影响因子：
1.8
通讯作者：
Liang, Zhiqing
中科院分区：
文献类型：
--
作者：
Liu, Lubin;Deng, Li;Liang, Zhiqing
本研究采用猪膀胱基质（UBM）对聚丙烯阴道外科材料进行改性，以提高其生物相容性。目的是开发一种复合支架，诱导较少的阴道糜烂，并评估这种材料在vivo.48 Sprague-Dawley大鼠随机分为四个相等的组宿主免疫反应。一组进行假手术，其他组进行不同材料的阴道植入：UBM（U）; UBM +聚丙烯（UP）;或聚丙烯（P）。术后7、14、21、28 d，通过大体观察、组织学和免疫组化方法观察宿主组织反应，P组在整个观察时间内炎症反应最强，U组和UP组较弱，并有随时间推移而减弱的趋势。复合支架中UBM材料的存在允许聚丙烯与新增殖的周围组织融合，并导致宿主对材料的排斥减少，如CD 4-和CD 8-阳性细胞的出现减少所示。猪UBM允许聚丙烯的机械分离，也减少了对聚丙烯的免疫反应。本研究提示UBM +聚丙烯复合支架可能是一种有前途的临床骨盆重建材料。
The present study set out to modify polypropylene vaginal surgical material using porcine urinary bladder matrix (UBM) in order to improve biocompatibility. The aim was to develop a compound scaffold that induced less vaginal erosion and to evaluate host immunoreactivity to this material in vivo.Forty-eight Sprague-Dawley rats were randomly divided into four equal groups. One group underwent a sham operation, and the other groups underwent vaginal implantation with different materials: UBM (U); UBM + polypropylene (UP); or polypropylene (P). The host tissue response was determined by macro-observation, and by histological and immunohistochemical methods at 7, 14, 21, or 28 days after surgery.The inflammation reaction was strongest throughout the entire observation time in Group P, but was weaker and had a tendency to decrease with time in Groups U and UP. The presence of the UBM material in the compound scaffold allowed the polypropylene to fuse with newly proliferating surrounding tissue and resulted in less rejection of the material by the host, as indicated by the reduced appearance of CD4-, and CD8-positive cells.Porcine UBM allowed mechanical isolation of polypropylene, and also reduced the immune reaction to polypropylene. This study suggests that the UBM + polypropylene compound scaffold may be a promising material for clinical use in pelvic reconstruction surgery.