High-Density Genetic Linkage Maps Provide Novel Insights Into ZW/ZZ Sex Determination System and Growth Performance in Mud Crab (Scylla paramamosain)
High-Density Genetic Linkage Maps Provide Novel Insights Into ZW/ZZ Sex Determination System and Growth Performance in Mud Crab (Scylla paramamosain)
复制标题
高密度遗传连锁图谱为青蟹（Scylla paramamosain）的 ZW/ZZ 性别决定系统和生长性能提供了新的见解
DOI：
10.3389/fgene.2019.00298
复制
发表时间：
2019-04-05
影响因子：
3.7
通讯作者：
Ma, Hongyu
中科院分区：
文献类型：
--
作者：
Waiho, Khor;Shi, Xi;Ma, Hongyu
拟穴青蟹（Scylla paramamosain）是全球水产养殖中最重要的甲壳类物种之一。目的探讨中华绒螯蟹性别和生长相关性状的遗传基础。利用SLAF-seq和一个全同胞家系构建了一个具有16，701个单核苷酸多态性（SNP）的高密度遗传连锁图谱。共有连锁群49个，总长度为5996.66 cM，平均标记间距为0.81 cM。在LG 32上共检测到516个SNP标记，其中8个为雌性特异性SNP，分布在两个表型性别的QTL上。雌性特异的SNP标记仅在雌性连锁图上存在，它们的分离模式和较低的雌性：雄性重组率强烈暗示了S. ZW/77性别决定系统的构造。拟穴龙大部分（90%）生长相关性状的QTL位于LG 46上较小的区间（25.18-33.74 cM）内，表明LG 46可能参与了生长相关的遗传。LG 46上的4个标记与10-16个生长相关性状显著相关。BW仅与标记3846相关。根据转录组数据的注释，在性别和生长相关性状的QTL区域内分别鉴定出11个和2个候选基因。新构建的高密度遗传连锁图谱和已鉴定的性别和生长相关性状的QTL，为甲壳动物分子标记辅助选择和遗传改良提供了宝贵的遗传资源和坚实的基础。
Mud crab, Scylla paramamosain is one of the most important crustacean species in global aquaculture. To determine the genetic basis of sex and growth-related traits in S. paramamosain, a high-density genetic linkage map with 16,701 single nucleotide polymorphisms (SNPs) was constructed using SLAF-seq and a full-sib family. The consensus map has 49 linkage groups, spanning 5,996.66 cM with an average marker-interval of 0.81 cM. A total of 516 SNP markers, including 8 female-specific SNPs segregated in two quantitative trait loci (QTLs) for phenotypic sex were located on LG32. The presence of female-specific SNP markers only on female linkage map, their segregation patterns and lower female: male recombination rate strongly suggest the conformation of a ZW/77 sex determination system in S. paramamosain. The QTLs of most (90%) growth-related traits were found within a small interval (25.18-33.74 cM) on LG46, highlighting the potential involvement of LG46 in growth. Four markers on LG46 were significantly associated with 10-16 growth-related traits. BW was only associated with marker 3846. Based on the annotation of transcriptome data, 11 and 2 candidate genes were identified within the QTL regions of sex and growth-related traits, respectively. The newly constructed high-density genetic linkage map with sex-specific SNPs, and the identified QTLs of sex- and growth-related traits serve as a valuable genetic resource and solid foundation for marker-assisted selection and genetic improvement of crustaceans.