Expressional alterations of fibrillin-1 during wound healing of human dental pulp
Expressional alterations of fibrillin-1 during wound healing of human dental pulp
复制标题
人牙髓伤口愈合过程中fibrillin-1的表达变化
DOI：
10.1016/j.joen.2011.09.016
复制
发表时间：
2012
期刊：
影响因子：
4.2
通讯作者：
et al
中科院分区：
文献类型：
--
作者：
Yoshiba N;et al
微原纤维的主要成分纤维蛋白的降解促进了活性转化生长因子-β(转化生长因子-β)的释放，这是一种信号分子，有助于在暴露的牙髓中形成矿化组织屏障。为了研究纤维蛋白在屏障形成中的作用，我们检测了(1)纤维蛋白基因和蛋白以及(2)可能与纤维蛋白降解和细胞分化相关的因子在暴露的人牙髓中的时空表达。人类牙髓切片培养也检测了纤维蛋白在矿化中的作用。方法对临床健康的牙髓进行机械暴露，并用三氧化二矿物团聚体覆盖。7~42d后取牙进行纤维蛋白-1、纤维蛋白-2、潜在转化生长因子-β结合蛋白-1、基质金属蛋白酶-3、碱性磷酸酶免疫组织化学染色及纤维蛋白-1的原位杂交。用β-甘油磷酸盐培养的牙髓组织切片，用免疫组织化学/细胞化学染色和定量逆转录聚合酶链式反应分析纤维蛋白-1、纤维蛋白-2和碱性磷酸酶。结果纤维蛋白-1免疫反应在暴露部位下方的牙髓区域一直持续到第7天，但从第14天开始消失。第14天检测到一过性的MMP3免疫反应。在42天矿化屏障明显时，纤维蛋白-1的免疫反应和纤维蛋白-1的表达仍然下调。纤维蛋白-2、LTBP-1和碱性磷酸酶在纤维蛋白-1检测不到的区域不断被检测到。β-甘油磷酸盐培养的牙髓切片显示矿化，碱性磷酸酶表达上调，纤维蛋白-1表达下调。结论暴露牙髓在体内矿化组织屏障形成和β-甘油磷酸诱导的体外牙髓矿化过程中，纤维蛋白-1的表达发生了降解和下调。
INTRODUCTIONThe degradation of fibrillins, the major constituents of microfibrils, is known to facilitate the release of active transforming growth factor-β (TGF-β), a signaling molecule contributing to mineralized tissue barrier formation in exposed dental pulps. To examine the involvement of fibrillins in the barrier formation, we examined the temporospatial expression of (1) genes and proteins of fibrillins and (2) factors possibly associated with fibrillin degradation and cytodifferentiation in exposed human pulps. Human pulp slice cultures were also examined for the role of fibrillins in mineralization.METHODSClinically healthy pulps were mechanically exposed and capped with mineral trioxide aggregate. After 7 to 42 days, the teeth were processed for immunohistochemical and cytochemical staining of fibrillin-1, fibrillin-2, latent TGF-β-binding protein (LTBP)-1, matrix metalloproteinase-3 (MMP-3), alkaline phosphatase (ALP), and in situ hybridization of fibrillin-1. Pulp tissue slices cultured with β-glycerophosphate were analyzed for fibrillin-1, fibrillin-2, and ALP with the immunohistochemical/cytochemical staining and quantitative reverse-transcriptase polymerase chain reaction.RESULTSFibrillin-1-immunoreactivity was seen until 7 days but turned into undetectable since 14 days in the pulpal area just beneath the exposure site. MMP-3-immunoreaction was transiently detected at 14 days. At 42 days when the mineralized barrier was evident, fibrillin-1-immunoreactivity and fibrillin-1 expression remained down-regulated. Fibrillin-2, LTBP-1, and ALP were constantly detected in the fibrillin-1–undetectable area. Pulp slices cultured with β-glycerophosphate showed mineralization with up-regulation of ALP and down-regulation of fibrillin-1.CONCLUSIONSDegradation and down-regulation of fibrillin-1 expression took place during the mineralized tissue barrier formation in exposed pulps in vivo and β-glycerophosphate–induced pulpal mineralization in vitro.