Lens metabolic cooperation: a study of mouse lens transport and permeability visualized with freeze-substitution autoradiography and electron microscopy.
Lens metabolic cooperation: a study of mouse lens transport and permeability visualized with freeze-substitution autoradiography and electron microscopy.
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DOI：
10.1083/jcb.86.2.576
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发表时间：
1980-08
期刊：
影响因子：
--
通讯作者：
Lyons JE
中科院分区：
文献类型：
--
作者：
Goodenough DA;Dick JS 2nd;Lyons JE
通过冷冻置换放射自显影术证明了代谢物在成年小鼠透镜隔室之间的转运。赖氨酸掺入的体内模式与赖氨酸、葡萄糖、尿苷和脱氧葡萄糖掺入的体外模式进行比较。细胞内和细胞外分布的氚代代谢产物的确定通过比较运输基板与非运输分子相似的分子大小：甘露醇和蔗糖。透镜细胞间隙的渗透性在荧光显微镜水平上用Procion Yellow探测，在电子显微镜水平上用辣根过氧化物酶探测。冷冻断裂电子显微镜显示上皮细胞之间、透镜纤维之间以及上皮细胞和透镜纤维之间的缝隙连接。在小鼠上皮细胞之间未常规观察到封闭带（紧密连接）。然而，后一种结果受种属差异的影响，因为鸡上皮细胞之间存在丰富的闭合小带。渗透性结果表明，透镜细胞能够代谢合作，广泛的间隙连接网络介导。
Transport of metabolites is demonstrated between compartments of the adult mouse lens by freeze-substitution autoradiography. In vivo patterns of lysine incorporation are compared with in vitro patterns of lysine, glucose, uridine, and deoxyglucose incorporation. Intracellular and extracellular distributions of tritiated metabolites are determined by comparison of transported substrates with the nontransported molecules of similar molecular size: mannitol and sucrose. The permeability of the lens intercellular spaces is probed with Procion Yellow at the level of fluorescence microscopy, and with horseradish peroxidase at the electron microscope level. Freeze-fracture electron microscopy reveals gap junctions between epithelial cells, between lens fibers, and between epithelial cells and lens fibers. Zonulae occludentes (tight junctions) are not routinely observed between epithelial cells in the mouse. This latter result is subject to species variation, however, since zonulae occludentes are abundant between chicken epithelial cells. The permeability results suggest that the lens cells are capable of metabolic cooperation, mediated by an extensive gap junction network.