First evidence for existence of an uphill electron transfer through the bc1 and NADH-Q oxidoreductase complexes of the acidophilic obligate chemolithotrophic ferrous ion-oxidizing bacterium Thiobacillus ferrooxidans
First evidence for existence of an uphill electron transfer through the bc1 and NADH-Q oxidoreductase complexes of the acidophilic obligate chemolithotrophic ferrous ion-oxidizing bacterium Thiobacillus ferrooxidans
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DOI：
10.1128/jb.182.12.3602-3606.2000
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发表时间：
2000-06-01
影响因子：
3.2
通讯作者：
Lemesle-Meunier, D
中科院分区：
文献类型：
--
作者：
Elbehti, A;Brasseur, G;Lemesle-Meunier, D
利用铁细胞色素c作为电子供体，研究了嗜酸性化学无机营养生物氧化亚铁硫杆菌（Thiobacillus ferrooxidans）中吡啶核苷酸还原过程中的能量依赖型电子转移途径.实验结果表明，这一上坡途径涉及bc（1）和NADH-Q氧化还原酶复合物的反向功能，使用ATP水解产生的电化学质子梯度。基于这些结果，提出了一个模型来解释从铁细胞色素c之间的放能和吸能电子转移途径的还原当量的平衡。
The energy-dependent electron transfer pathway involved in the reduction of pyridine nucleotides which is required for CO2 fixation to occur in the acidophilic chemolithotrophic organism Thiobacillus ferrooxidans was investigated using ferrocytochrome c as the electron donor. The experimental results show that this uphill pathway involves a bc(1) and an NADH-Q oxidoreductase complex functioning in reverse, using an electrochemical proton gradient generated by ATP hydrolysis. Based on these results, a model is presented to explain the balance of the reducing equivalent from ferrocytochrome c between the exergonic and endergonic electron transfer pathways.