TEMPORAL INFILTRATION OF LEUKOCYTE SUBSETS INTO MOUSE SKIN INFLAMED WITH PHORBOL ESTER
TEMPORAL INFILTRATION OF LEUKOCYTE SUBSETS INTO MOUSE SKIN INFLAMED WITH PHORBOL ESTER
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DOI：
10.1007/bf02028118
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发表时间：
1992-11-01
期刊：
影响因子：
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通讯作者：
TRAMPOSCH, KM
中科院分区：
文献类型：
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作者：
ALFORD, JG;STANLEY, PL;TRAMPOSCH, KM
我们之前的研究表明，在超过11天的时间内，多次局部应用酚酯12- o -十四烷基酚-13-醋酸酯（TPA）可诱导以水肿、细胞浸润和表皮增生为特征的持续炎症反应。为了表征炎症反应建立过程中的细胞浸润，使用两种单克隆抗体：巨噬细胞抗体MOMA-2和泛t细胞抗体Thy-1进行免疫组化。多形核白细胞（PMNs）水平在第3天达到峰值，为未处理对照组的160倍，然后在第7-10天下降至30倍。到第4天，巨噬细胞数量比未处理的对照组增加了2.9倍，到第10天，巨噬细胞数量比未处理的对照组增加了6.0倍。相比之下，与未治疗组相比，第7天存在的t细胞数量显着增加了9.5倍，在第8天达到峰值，与未治疗组相比增加了19倍。用戊酸氢化可的松局部治疗动物导致组织中存在的t细胞数量急剧减少（约60%）。相反，类固醇对组织中巨噬细胞的数量没有影响。特异性细胞类型的鉴定及其浸润的时间过程与慢性炎性病变的发展是一致的。
We have previously shown that multiple topical applications, over 11 days, of the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) induces a persistent inflammatory reaction characterized by edema, cell infiltration and epidermal hyperplasia. In order to characterize the cell infiltrate during the establishment of this inflammatory reaction, immunohistochemistry was performed using two monoclonal antibodies: MOMA-2, a macrophage antibody and Thy-1, a pan T-cell antibody. The level of polymorphonuclear leukocytes (PMNs) peaked by day 3 at 160-fold over nontreated controls and then subsided to a 30-fold elevation on days 7-10. By day 4, the number of macrophages increased 2.9-fold over the nontreated control and by day 10 were elevated 6.0-fold over the nontreated control. In comparison, the number of T-cells present by day 7 was significantly elevated 9.5-fold over the nontreated group and peaked at day 8 with a 19-fold elevation relative to nontreated controls. Topical treatment of animals with hydrocortisone valerate resulted in a dramatic ( > 60%) reduction in the number of T-cells present in the tissue. In contrast, there was no effect of the steroid on the number of macrophages present in the tissue. The identification of specific cell types and their time course of infiltration is consistent with the development of a chronic inflammatory lesion.