Ophiopogon polysaccharide liposome can enhance the non-specific and specific immune response in chickens
Ophiopogon polysaccharide liposome can enhance the non-specific and specific immune response in chickens
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麦冬多糖脂质体增强鸡非特异性和特异性免疫反应
DOI：
10.1016/j.carbpol.2014.11.048
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发表时间：
2015-03-30
影响因子：
11.2
通讯作者：
Tong, Dewen
中科院分区：
文献类型：
--
作者：
Fan, Yunpeng;Ma, Xia;Tong, Dewen
研究麦冬多糖脂质体（OPL）的免疫增强作用。在非特异性免疫应答实验中，观察了小鼠腹腔巨噬细胞在体内外的吞噬功能和细胞因子的分泌情况。在特异性免疫应答试验中，用纽卡斯尔疫苗免疫鸡，49日龄时用新城疫病毒攻毒，测定OPL活性。结果表明，OPL在体外能显著促进巨噬细胞吞噬功能，诱导IL-2和IL-6的分泌;在体内，OPL高、中剂量能显著提高巨噬细胞吞噬指数，促进淋巴细胞增殖，提高T淋巴细胞亚群（CD 4（+）和CD 8（+））比例，增强抗体滴度，提高保护率。其药效明显优于麦冬多糖（OP）。结果表明，OP经脂质体包封后，其免疫增强活性明显提高。(C)2014爱思唯尔有限公司版权所有。
The purpose of this study is to investigate the immune-enhancing activity of ophiopogon polysaccharide liposome (OPL). In non-specific immune response experiment, the phagocytosis and cytokines secretion of peritoneal macrophages in vitro and in vivo were performed. In specific immune response experiment, the activity of OPL was measured on chickens which were vaccinated with Newcastle disease (ND) vaccine and then challenged with ND virus at 49-old-day. The results showed that OPL could significantly promote the phagocytosis of macrophages and induce the secretion of IL-2 and IL-6 in vitro; OPL at high and medium doses could significantly improve the phagocytosic index, promote lymphocyte proliferation, increase the proportion of T lymphocyte subpopulations (CD4(+) and CD8(+)), enhance antibody titer and improve the protective rate in vivo. Moreover, its efficacy was significantly better than ophiopogon polysaccharide (OP). These results indicated that the immune-enhancing activity of OP was significantly improved after encapsulated with liposome. (C) 2014 Elsevier Ltd. All rights reserved.