PROSTAGLANDIN AND THROMBOXANE SYNTHESIS BY RAT GLOMERULAR EPITHELIAL-CELLS
PROSTAGLANDIN AND THROMBOXANE SYNTHESIS BY RAT GLOMERULAR EPITHELIAL-CELLS
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DOI：
10.1038/ki.1981.163
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发表时间：
1981-01-01
影响因子：
19.6
通讯作者：
DUNN, MJ
中科院分区：
文献类型：
--
作者：
PETRULIS, AS;AIKAWA, M;DUNN, MJ
离体大鼠肾小球合成前列腺素（PG）和甲状腺素（Tx）。用放射免疫分析法和放射色谱法测定培养的肾小球细胞合成PG和Tx的能力。透射电镜和扫描电镜显示多角形细胞，附着桥粒，表面有微绒毛。这些特征是肾小球上皮细胞的典型特征。将这些肾小球上皮细胞与花生四烯酸（C20：4）一起孵育，产生一系列终产物，其浓度为PGE 2> TxB 2> PGF 2 α。> 6-酮-PGF 1 α。向培养的肾小球细胞中加入血管紧张素II（AII）产生几乎排他的PGE 2刺激。PGF2.alpha. > TxB 2 = 6-酮-PGF 1 α。AII和AIII（100-1 μ M）刺激肾小球上皮细胞中的PGE 2，并且PGE 2的增量作为AII或AIII浓度的函数是相似的。Sar 1-Thr 8-AII类似物抑制AII和AIII刺激的PGE 2合成。浓度为0.2-2.0 μ M的二价阳离子离子载体A23187 [calcimycin]主要增加PGE 2和TxB 2的合成，而PGF 2 α的增加较小。和6-酮-PGF 1 α。用C20：4或A23187孵育的大鼠肾小球上皮细胞产生的PG和Tx的相对浓度相似。9天后，在大鼠肾小球培养物中生长的主要细胞显然是上皮细胞;该细胞能够合成PG和Tx; AII和AIII对PG的刺激可能由相同的细胞受体介导，AII和AIII主要增加血管舒张性PG PGE 2的合成;外源性底物C20：4或二价阳离子离子载体A23187释放内源性C20：4不仅刺激PGE 2，而且刺激血管收缩剂TxA 2;并且由上皮细胞合成的PG和Tx终产物可以通过特异性合成酶与不同的C20：4库的细胞内偶联来确定。
Isolated rat glomeruli synthesize prostaglandin (PG) and thyromboxane (Tx). By radioimmunoassay and radiochromatographic methods, PG and Tx synthesis by glomerular cells in culture were evaluated. Transmission and scanning electron microscopy showed polygonal cells, attached by desmosomes, with surface microvilli. These features are typical of glomerular epithelial cells. Incubation of these glomerular epithelial cells with arachidonic acid (C20:4) resulted in an array of endproducts with concentrations of PGE2 > TxB2 > PGF2.alpha. > 6-keto-PGF1.alpha.. Addition of angiotensin II (AII) to the cultured glomerular cell produced almost exclusive stimulation of PGE2 with PGE2 .mchgt. PGF2.alpha. > TxB2 = 6-keto-PGF1.alpha.. AII and AIII (100-1 .mu.M) stimulated PGE2 in glomerular epithelial cells, and the increments of PGE2, as a function of the concentration of AII or AIII, were similar. The Sar1-Thr8-AII analog inhibited both AII- and AIII-stimulated PGE2 synthesis. The divalent cation ionophore A23187 [calcimycin] in concentrations of 0.2-2.0 .mu.M increased primarily PGE2 and TxB2 synthesis with smaller increases of PGF2.alpha. and 6-keto-PGF1.alpha.. The relative concentrations of PG and Tx produced by rat glomerular epithelial cells, incubated with C20:4 or A23187, were similar. The predominant cell grown in culture from the rat glomerulus, after 9 days, evidently is the epithelial cell; this cell is capable of PG and Tx synthesis; stimulation of PG by AII and AIII may be mediated by the same cellular receptor, AII and AIII increase primarily the synthesis of a vasodilatory PG, PGE2; exogenous substrate C20:4 or release of endogenous C20:4 by the divalent cation ionophore A23187 not only stimulates PGE2 but also stimulates the vasocontrictor TxA2; and the PG and Tx endproducts synthesized by epithelial cells may be determined by an intracellular coupling of the specific synthetic enzymes with different pools of C20:4.