ADENOSINE-3',5'-MONOPHOSPHATE WAVES IN DICTYOSTELIUM-DISCOIDEUM - A DEMONSTRATION BY ISOTOPE DILUTION-FLUOROGRAPHY
ADENOSINE-3',5'-MONOPHOSPHATE WAVES IN DICTYOSTELIUM-DISCOIDEUM - A DEMONSTRATION BY ISOTOPE DILUTION-FLUOROGRAPHY
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DOI：
10.1126/science.6259734
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发表时间：
1981-01-01
期刊：
影响因子：
56.9
通讯作者：
DEVREOTES, PN
中科院分区：
文献类型：
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作者：
TOMCHIK, KJ;DEVREOTES, PN
采用新型同位素稀释-荧光技术研究了cAMP在盘状变形虫聚集区的分布。细胞cAMP通过与外源性3h标记的cAMP竞争蛋白激酶上的高亲和力结合位点来可视化，该蛋白激酶固定在用于印迹单层的Millipore过滤器上。cAMP以聚集灶为中心呈螺旋状或同心圆波分布。这些模式与通过单层传播的细胞形状变化相关：cAMP浓度高的区域的细胞被拉长（可能是沿着cAMP梯度向上移动）；cAMP浓度低的区域的细胞是随机定向的。cAMP的最高浓度为。10 ~ 6 m， cAMP升高区宽度约为。0.3-1毫米，假设波速为300亩。每分钟M表示一个细胞的信号为。1 - 3分钟。盘基骨鞘的聚集过程显然是由cAMP信号的周期性中继介导的，这为聚集过程的动力学提供了一个简单的方案。
The distribution of cAMP in fields of aggregating amoebae of D. discoideum was examined by a novel isotope dilution-fluorographic technique. Cellular cAMP was visualized by its competition with exogenous 3H-labeled cAMP for high-affinity binding sites on protein kinase immobilized on a Millipore filter used to blot the monolayer. The cAMP was distributed in spiral or concentric circular wave patterns which centered on the foci of the aggregations. These patterns were correlated with those of cell shape change that propagate through the monolayers: cells in regions of high concentrations of cAMP were elongated (presumably moving up a cAMP gradient; those in regions of low cAMP concentrations were randomly directed. The highest cAMP concentrations were .apprx. 10-6 M. The widths of the regions of elevated cAMP were .apprx. 0.3-1 mm, which, assuming a wave velocity of 300 .mu.M per min suggests that a cell signals for .apprx. 1-3 min. The aggregation process in Dictyostelium apparently is mediated by the periodic relay of cAMP signals and suggest a simple scheme for the dynamics of the aggregation process.