Adsorption of serum albumin to thin films of poly(lactide-co-glycolide)
Adsorption of serum albumin to thin films of poly(lactide-co-glycolide)
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DOI：
10.1016/s0168-3659(98)00173-4
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发表时间：
1999-04-19
影响因子：
10.8
通讯作者：
Tilton, RD
中科院分区：
文献类型：
--
作者：
Butler, SM;Tracy, MA;Tilton, RD
蛋白质吸附与可生物降解微球释放药物的可变性有关。我们用光学反射法测量了牛血清白蛋白（BSA）在两种具有不同端基（一个是亲水性羧基，另一个是疏水性酯端基）的聚乳酸-共聚物（PLG）样品制备的光滑自旋浇注膜上的吸附程度和动力学。我们中的一个先前已经表明，这些端基影响微球的降解（Tracy et al., 1998，影响聚（丙交酯-共聚物）微球在体内和体外降解率的因素）。生物材料：提交出版。)。两种膜均具有中等疏水性，其润湿性与端基类型无关。BSA很容易吸附到两种原生PLG薄膜上，达到高达50%的表面覆盖面积，并且对端基类型不敏感。在暴露于BSA之前，将膜在水中老化24小时降低了膜的疏水性，这反过来又与初始BSA吸附速率的显着降低相关。这与经常观察到的表面疏水性有利于蛋白质吸附的趋势是一致的。尽管降低的初始吸附速率显示出较低的吸附亲和力，但老化膜上的最终吸附量超过了天然膜上的吸附量，这可能是由于部分PLG侵蚀产生的总表面积增加。(C)1999 Elsevier Science BN。版权所有。
Protein adsorption has been implicated in the variability of drug release from biodegradable microspheres. We used optical reflectometry to measure the extent and kinetics of bovine serum albumin (BSA) adsorption to smooth spin-cast films prepared from two poly(lactide-co-glycolide) (PLG) samples that have different end-groups, one being a hydrophilic carboxylic end group and the other a hydrophobic ester end group. One of us has previously shown that these end-groups influence microsphere degradation (Tracy et al., 1998, Factors affecting the degradation rate of poly(lactide-co-glycolide) microspheres in vivo and in vitro. Biomaterials: submitted for publication.). Both films were moderately hydrophobic, and their wettability was independent of the type of end-group. BSA adsorbed readily to both native PLG films, attaining as much as 50% surface coverage by area and was insensitive to the type of end-group. Aging the films in water for 24 h prior to BSA exposure decreased the hydrophobicity of the films and this in turn correlated with a significant decrease in the initial BSA adsorption rate. This was consistent with the often-observed trend that surface hydrophobicity favors protein adsorption. In spite of the lower adsorption affinity revealed by this decreased initial adsorption rate, the final adsorbed amounts on the aged films exceeded those attained on native films, presumably due to the increase in total surface area produced by partial PLG erosion. (C)1999 Elsevier Science BN. All rights reserved.