CHARACTERIZATION OF THE MINOR GROOVE ENVIRONMENT IN A DRUG DNA COMPLEX - BISBENZIMIDE BOUND TO THE POLY[D(AT)].POLY[D(AT)]DUPLEX
CHARACTERIZATION OF THE MINOR GROOVE ENVIRONMENT IN A DRUG DNA COMPLEX - BISBENZIMIDE BOUND TO THE POLY[D(AT)].POLY[D(AT)]DUPLEX
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DOI：
10.1073/pnas.85.23.8939
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发表时间：
1988-12-01
影响因子：
11.1
通讯作者：
BRESLAUER, KJ
中科院分区：
文献类型：
--
作者：
JIN, R;BRESLAUER, KJ
我们比较了与聚[d(AT)] .cntdot的小凹槽结合的双苯并亚胺（也称为Hoechst 33258）的荧光特性。在纯有机溶剂和有机/水混合溶剂中溶解的具有相应荧光性质的双苯并亚胺聚[d(AT)]双相。基于这些比较，我们得出结论：双苯并亚胺-poly[d(AT)].cntdot.poly[d(AT)]配合物的小槽是非极性的，其局部介电常数为。apprxeq。20 D.我们讨论了这一见解如何影响我们对分子力的理解，这些分子力决定和控制小凹槽导向DNA结合配体的结合亲和力和特异性。
We compare the fluorescence properties of bisbenzimide (also known as Hoechst 33258) bound to the minor groove of the poly[d(AT)] .cntdot. poly[d(AT)] duplex with the corresponding fluorescence properties of bisbenzimide dissolved in neat organic solvents and mixed organic/aqueous solvents. Based on these comparisons, we conclude that the minor groove of the bisbenzimide-poly[d(AT)].cntdot.poly[d(AT)] complex is quite nonpolar and exhibits a local dielectric constant of .apprxeq. 20 D. We discuss how this insight influences our understanding of the molecular forces that dictate and control the binding affinities and specificities of minor groove-directed DNA binding ligands.