Comparison of the release of adipokines by adipose tissue, adipose tissue matrix, and Adipocytes from visceral and subcutaneous abdominal adipose tissues of obese humans
Comparison of the release of adipokines by adipose tissue, adipose tissue matrix, and Adipocytes from visceral and subcutaneous abdominal adipose tissues of obese humans
复制标题
DOI：
10.1210/en.2003-1336
复制
发表时间：
2004-05-01
期刊：
影响因子：
4.8
通讯作者：
Bahouth, SW
中科院分区：
文献类型：
--
作者：
Fain, JN;Madan, AK;Bahouth, SW
本研究的目的是探讨肥胖者内脏和皮下脂肪组织释放脂肪因子的来源。在原代培养物中孵育48小时的人脂肪组织释放的前列腺素E-2、IL-8和IL-6比脂联素多，而纤溶酶原激活物抑制剂1和肝细胞生长因子的释放量低于脂联素，但高于瘦素。IL-10和TNF α的释放量低于瘦素，而血管内皮生长因子和IL-β的释放量要低得多。还在通过脂肪组织的胶原酶消化获得的三种组分（脂肪组织基质、分离的基质血管细胞和脂肪细胞）中检查脂肪因子的积累。除脂联素和瘦素外，90%以上的脂肪组织释放的脂肪因子可归因于非脂肪细胞。内脏脂肪组织释放的血管内皮生长因子，IL-6，和纤溶酶原激活物抑制剂1与腹部皮下组织相比，更大量。与32相比，体重指数为45的个体脂肪组织释放TNF α、IL-8和IL-10的总量大大增加，这是由于非脂肪细胞。此外，大多数脂肪组织的非脂肪细胞释放的脂肪因子是由于胶原酶消化后保留在组织基质中的细胞。
The purpose of this study was to examine the source of adipokines released by the visceral and sc adipose tissues of obese humans. Human adipose tissue incubated in primary culture for 48 h released more prostaglandin E-2, IL-8, and IL-6 than adiponectin, whereas the release of plasminogen activator inhibitor 1 and hepatocyte growth factor was less than that of adiponectin but greater than that of leptin. IL-10 and TNFalpha were released in amounts less than those of leptin, whereas vascular endothelial growth factor and IL-beta were released in much lower amounts. The accumulation of adipokines was also examined in the three fractions (adipose tissue matrix, isolated stromovascular cells, and adipocytes) obtained by collagenase digestion of adipose tissue. Over 90% of the adipokine release by adipose tissue, except for adiponectin and leptin, could be attributed to nonfat cells. Visceral adipose tissue released greater amounts of vascular endothelial growth factor, IL-6, and plasminogen activator inhibitor 1 compared with abdominal sc tissue. The greatly enhanced total release of TNFalpha, IL-8, and IL10 by adipose tissue from individuals with a body mass index of 45 compared with 32 was due to nonfat cells. Furthermore, most of the adipokine release by the nonfat cells of adipose tissue was due to cells retained in the tissue matrix after collagenase digestion.