Alternative Splicing Mediates Responses of the Arabidopsis Circadian Clock to Temperature Changes
Alternative Splicing Mediates Responses of the Arabidopsis Circadian Clock to Temperature Changes
复制标题
DOI：
10.1105/tpc.111.093948
复制
发表时间：
2012-03-01
期刊：
影响因子：
11.6
通讯作者：
Nimmo, Hugh G.
中科院分区：
文献类型：
--
作者：
James, Allan B.;Syed, Naeem Hasan;Nimmo, Hugh G.
选择性剪接通过影响转录组和蛋白质组的多样性，调节蛋白质结构/功能和基因表达而发挥重要作用。它广泛存在于植物中，剪接因子水平的改变导致了各种各样的生长和发育表型。生物钟是一种复杂的细胞机制，可以调节生理和行为，以预测旋转星球上可预测的环境变化。我们对适应不同稳态温度或经历温度转变的拟南芥植物的时钟组件进行了全系统的选择性剪接分析。这揭示了时钟基因中广泛的选择性剪接和选择性剪接转录本的动态变化。其中一些变化，特别是影响生物钟基因LATE ELONGATED HYPOCOTYL （LHY）和PSEUDO RESPONSE REGULATOR7的变化，是温度依赖性的，并通过产生无功能转录物和/或诱导无义介导的衰变，显著地促进了温度转变中生物钟基因表达的重要功能变化。温度对选择性剪接的影响导致LHY转录物丰度在冷却时下降，但LHY启动子强度不受影响。我们认为，温度相关的选择性剪接是参与植物生物钟运作和调节的另一种机制。
Alternative splicing plays crucial roles by influencing the diversity of the transcriptome and proteome and regulating protein structure/function and gene expression. It is widespread in plants, and alteration of the levels of splicing factors leads to a wide variety of growth and developmental phenotypes. The circadian clock is a complex piece of cellular machinery that can regulate physiology and behavior to anticipate predictable environmental changes on a revolving planet. We have performed a system-wide analysis of alternative splicing in clock components in Arabidopsis thaliana plants acclimated to different steady state temperatures or undergoing temperature transitions. This revealed extensive alternative splicing in clock genes and dynamic changes in alternatively spliced transcripts. Several of these changes, notably those affecting the circadian clock genes LATE ELONGATED HYPOCOTYL (LHY) and PSEUDO RESPONSE REGULATOR7, are temperature-dependent and contribute markedly to functionally important changes in clock gene expression in temperature transitions by producing nonfunctional transcripts and/or inducing nonsense-mediated decay. Temperature effects on alternative splicing contribute to a decline in LHY transcript abundance on cooling, but LHY promoter strength is not affected. We propose that temperature-associated alternative splicing is an additional mechanism involved in the operation and regulation of the plant circadian clock.