The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. II. The GC-rich region
The nucleotide sequence of oocyte 5S DNA in Xenopus laevis. II. The GC-rich region
复制标题
非洲爪蟾卵母细胞 5S DNA 的核苷酸序列。
DOI：
10.1016/0092-8674(78)90221-0
复制
发表时间：
1978
期刊：
影响因子：
64.5
通讯作者：
Donald D. Brown
中科院分区：
文献类型：
--
作者：
J. Miller;E. Cartwright;G. Brownlee;N. Fedoroff;Donald D. Brown
在单个质粒克隆的重复单元和整个重复单元群中测定了X. laevis 5s DNA中富含gc的重复单元的一半的一级序列。富含gc的重复单元的一半包含174个核苷酸的长重复。复制片段在基因5 '端前73个核苷酸处开始，在基因101个核苷酸处终止。基因的复制部分，称为假基因，与基因的相应部分相差10个核苷酸，其余73个核苷酸的复制序列相差13个核苷酸。质粒克隆的重复单元与重复单元总数中的优势序列在富gc区有6个核苷酸的差异。有证据表明，大多数DNA中的CpG二核苷酸至少部分甲基化。
The primary sequence of the GC-rich half of the repeating unit in X. laevis 5s DNA has been determined in both a single plasmid-cloned repeating unit and in the total population of repeating units. The GC-rich half of the repeating unit contains a single long duplication of 174 nucleotides. The duplicated segment commences 73 nucleotides preceding the 5’end of the gene and terminates at nucleotide 101 of the gene. The duplicated portion of the gene, termed the pseudogene, differs by 10 nucleotides from the corresponding portion of the gene, and the remaining duplicated sequence of 73 nucleotides differs by 13 nucleotides. The plasmid-cloned repeating unit differs from the dominant sequence in the total population of repeating units by 6 nucleotides in the GC-rich region. Evidence is provided that most of the CpG dinucleotides in 5s DNA are at least partially methylated.