PROPERTIES OF INCOMPLETE SENDAI VIRIONS AND SUBGENOMIC VIRAL RNAS
PROPERTIES OF INCOMPLETE SENDAI VIRIONS AND SUBGENOMIC VIRAL RNAS
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DOI：
10.1016/0042-6822(70)90335-1
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发表时间：
1970-01-01
期刊：
影响因子：
3.7
通讯作者：
DARLINGTON, RW
中科院分区：
文献类型：
--
作者：
KINGSBURY, DW;PORTNER, A;DARLINGTON, RW
一株仙台病毒除含有50个S RNA基因组外，还含有19个S RNA和25个S RNA。19株S和25株S与仙台病毒感染细胞特异性RNA杂交，而与50株S病毒无杂交，表明它们含有与50株S病毒基因组相似的核苷酸序列。用速率区带离心法将仙台病毒粒子分成沉积温度约为400 S、1000 S和大于1000的S病毒粒子。在S或更高的温度下沉积的病毒粒子具有感染性，含有19个S、25个S和50个S的RNA。400个S病毒粒子中仅含有19个S和25个S RNA，不具感染性，干扰了感染性病毒粒子的复制。仙台病毒低度传代后，子代缺失S病毒400个，S 19个，S 25个。证据表明，仙台病毒复制过程中存在含有不完全基因组的非感染性病毒粒子。
A strain of Sendai virus encapsidated 19 S and 25 S RNAs in addition to 50 S RNA genomes. The 19 S and 25 S RNAs hybridized with Sendai virus-specific RNAs from infected cells, but not with 50 S viral RNA, indicating that they contained nucleotide sequences similar to 50 S viral genomes. Sendai virions were separated by rate zonal centrifugation into groups sedimenting at about 400 S, 1000 S, and greater than 1000 S. Virions sedimenting at 1000 S or greater were infectious and contained 19 S, 25 S, and 50 S RNAs. The 400 S virions contained only 19 S and 25 S RNAs; they were not infectious, and they interfered with replication of infectious virions. Passage of Sendai virus at low multiplicity gave progeny lacking 400 S virions and 19 S and 25 S RNAs. The evidence indicates that noninfectious virions containing incomplete genomes occur in Sendai virus replication.