Adipose tissue production of hepatocyte growth factor contributes to elevated serum HGF in obesity
Adipose tissue production of hepatocyte growth factor contributes to elevated serum HGF in obesity
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DOI：
10.1152/ajpendo.00174.2006
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发表时间：
2006-10-01
影响因子：
5.1
通讯作者：
Considine, Robert V.
中科院分区：
文献类型：
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作者：
Bell, Lauren N.;Ward, Jennifer L.;Considine, Robert V.
肥胖个体的血清 HGF 升高。这项研究探讨了肥胖患者体内过多的脂肪组织对循环 HGF 水平增加的影响。在减肥手术或 24 小时禁食导致体重减轻之前和之后，通过 ELISA 测量血清 HGF。术后 6.1 +/- 0.1 个月时，BMI（50.6 +/- 1.6 对比 35.1 +/- 1.3 kg/m(2)；P < 0.0001）和血清 HGF 显着降低（1,164 +/- 116 对比 529 +/- 39 pg/ml，P < 0.001）。 24 小时禁食不会改变血清 HGF，但血清瘦素显着降低（67.7 +/- 7.1 与 50.3 +/- 8.3 ng/ml，P = 0.02）。肥胖（BMI 40.3 +/- 2.8 kg/m(2)）受试者脂肪细胞分泌的 HGF 体外分泌量显着高于瘦受试者（BMI 23.3 +/- 1.4 kg/m(2)）受试者脂肪细胞的释放量（80.9 +/- 10.4 对比 21.5 +/- 4.0 pg/10(5) 细胞，P = 0.008）。通过实时 RT-PCR 测定的 HGF mRNA 水平在瘦人 (BMI 24.0 +/- 0.8 kg/m(2)) 和肥胖 (45.7 +/- 3.0 kg/m(2)) 受试者的脂肪细胞中没有差异，但在所研究的肥胖个体中血清 HGF 显着升高（787 +/- 61 与 489 +/- 49 pg/ml，P = 0.001）。 TNF-α（24 小时处理）显着增加皮下脂肪细胞的 HGF 释放，比对照显着增加 23.6 +/- 8.3%（P = 0.02）。这些数据表明，肥胖症患者血清 HGF 升高的部分原因是脂肪组织过多，并且可以通过减肥减少脂肪组织质量来逆转这种效应。肥胖受试者脂肪细胞的 HGF 分泌增加可能是由于可能与脂肪细胞大小和肥胖个体脂肪组织中升高的 TNF-α 刺激有关的转录后事件。
Serum HGF is elevated in obese individuals. This study examined the contribution of excess adipose tissue to increased circulating HGF levels in obesity. Serum HGF was measured by ELISA before and after weight loss due to bariatric surgery or a 24-h fast. At 6.1 +/- 0.1 mo following surgery, BMI (50.6 +/- 1.6 vs. 35.1 +/- 1.3 kg/m(2); P < 0.0001) and serum HGF were significantly decreased (1,164 +/- 116 vs. 529 +/- 39 pg/ml, P < 0.001). A 24-h fast did not change serum HGF, but serum leptin was significantly reduced (67.7 +/- 7.1 vs. 50.3 +/- 8.3 ng/ml, P = 0.02). HGF secretion in vitro from adipocytes of obese (BMI 40.3 +/- 2.8 kg/m(2)) subjects was significantly greater (80.9 +/- 10.4 vs. 21.5 +/- 4.0 pg/10(5) cells, P = 0.008) than release from adipocytes of lean (BMI 23.3 +/- 1.4 kg/m(2)) subjects. HGF mRNA levels determined by real-time RT-PCR were not different in adipocytes from lean (BMI 24.0 +/- 0.8 kg/m(2)) and obese (45.7 +/- 3.0 kg/m(2)) subjects, but serum HGF was significantly elevated in the obese individuals studied (787 +/- 61 vs. 489 +/- 49 pg/ml, P = 0.001). TNF-alpha (24 h treatment) significantly increased HGF release from subcutaneous adipocytes 23.6 +/- 8.3% over control (P = 0.02). These data suggest that elevated serum HGF in obesity is in part attributable to excess adipose tissue and that this effect can be reversed by reducing adipose tissue mass through weight loss. Increased HGF secretion from adipocytes of obese subjects may be due to posttranscriptional events possibly related to adipocyte size and stimulation by elevated TNF-alpha in the adipose tissue of obese individuals.