Optimized Media Volumes Enable Homogeneous Growth of Mesenchymal Stem Cell-Based Engineered Cartilage Constructs.
Optimized Media Volumes Enable Homogeneous Growth of Mesenchymal Stem Cell-Based Engineered Cartilage Constructs.
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优化的培养基体积使基于间充质干细胞的工程软骨结构能够均匀生长。
DOI：
10.1089/ten.tea.2020.0123
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发表时间：
2021
影响因子：
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通讯作者：
Mauck,RobertL
中科院分区：
文献类型：
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作者：
Zlotnick,HannahM;Stoeckl,BrendanD;Henning,ElizabethA;Steinberg,DavidR;Mauck,RobertL
尽管软骨组织工程领域取得了显著进展，但要使软骨结构具有均匀性仍然是一个挑战。此外，为了使工程软骨进入临床，这种均匀生长必须以一种高效的方式进行。在这项研究中，我们研究了随着时间的推移，增加培养基容量加速间充质干细胞（MSC）负载工程构建物均匀成熟的潜力。在4周和8周的软骨培养后，我们使用大量的机械、组织学和生化指标来评估msc负载构建物。这些分析是在完整的总结构和结构核心上进行的，以阐明区域依赖性差异。此外，还评估了局部应变传递，以量化整个结构中与深度相关的力学性能。我们的研究结果表明，培养基体积的增加可以在培养早期促进基质沉积，并在后期改善不必要的区域异质性。综上所述，这些数据支持在体外培养过程中使用更高的培养基量来加速组织成熟并增加组织结构的核心强度。这些发现将推动软骨组织工程领域的发展和组织工程构建的转化。
Despite marked advances in the field of cartilage tissue engineering, it remains a challenge to engineer cartilage constructs with homogeneous properties. Moreover, for engineered cartilage to make it to the clinic, this homogeneous growth must occur in a time-efficient manner. In this study we investigated the potential of increased media volume to expedite the homogeneous maturation of mesenchymal stem cell (MSC) laden engineered constructs over timein vitro. We assessed the MSC-laden constructs after 4 and 8 weeks of chondrogenic culture using bulk mechanical, histological, and biochemical measures. These assays were performed on both the intact total constructs and the construct cores to elucidate region-dependent differences. In addition, local strain transfer was assessed to quantify depth-dependent mechanical properties throughout the constructs. Our findings suggest that increased media volume enhances matrix deposition early in culture and ameliorates unwanted regional heterogeneities at later time points. Taken together, these data support the use of higher media volumes duringin vitroculture to hasten tissue maturation and increase the core strength of tissue constructs. These findings will forward the field of cartilage tissue engineering and the translation of tissue engineered constructs.