METHYLATED, BLOCKED 5' TERMINI IN HELA-CELL MESSENGER-RNA
METHYLATED, BLOCKED 5' TERMINI IN HELA-CELL MESSENGER-RNA
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DOI：
10.1073/pnas.72.5.1904
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发表时间：
1975-01-01
影响因子：
11.1
通讯作者：
DARNELL, JE
中科院分区：
文献类型：
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作者：
FURUICHI, Y;MORGAN, M;DARNELL, JE
由[甲基-(3)H]蛋氨酸或[(32)P]磷酸盐标记的细胞制备的Poly(A)-containing HeLa细胞mRNA被发现含有多种甲基化，阻断两种类型的5'端结构：m(7)GpppN(7)- np和m(7)GpppN(m)- n (m)- np。此外，N(6)-甲基腺苷中约有三分之一的[(3)H]甲基标签存在；这个标记的核苷在mRNA链的3‘端三分之一处没有发现，因此可能也在mRNA的5’部分。
Poly(A)-containing HeLa cell mRNA prepared from cells labeled with [methyl-(3)H]methionine or [(32)P]phosphate was found to contain a variety of methylated, blocked 5'-terminal structures of two general types: m(7)GpppN(7)-Np and m(7)GpppN(m)-N(m)-Np. In addition, about one-third of the [(3)H]methyl label was present in the N(6)-methyladenosine; this labeled nucleoside was not found in the 3'-terminal one-third of the mRNA chain and thus may also be in the 5' portion of the mRNA.