Preparation of rodent primary cultures for neuron-glia, mixed glia, enriched microglia, and reconstituted cultures with microglia.
Preparation of rodent primary cultures for neuron-glia, mixed glia, enriched microglia, and reconstituted cultures with microglia.
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DOI：
10.1007/978-1-62703-520-0_21
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Hong JS
中科院分区：
文献类型：
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作者：
Chen SH;Oyarzabal EA;Hong JS
小胶质细胞和大胶质细胞（星形胶质细胞和少突胶质细胞）是中枢神经系统中的主要细胞类型。小胶质细胞与其他脑细胞，神经元 - glia，神经元 - 胶质细胞和混合神经胶质培养之间的相互作用。适应性的方案，用于从啮齿动物中制备这些含小胶质细胞的原发性培养物。
Microglia, neurons, and macroglia (astrocytes and oligodendrocytes) are the major cell types in the central nervous system. In the past decades, primary microglia-enriched cultures have been widely used to study the biological functions of microglia in vitro. In order to study the interactions between microglia and other brain cells, neuron–glia, neuron–microglia, and mixed glia cultures were developed. The aim of this chapter is to provide basic and adaptable protocols for the preparation of these microglia-containing primary cultures from rodent. Meanwhile, we also want to provide a collection of tips from our collective experiences doing primary brain cell cultures.