A SATELLITE RNA OF BARLEY YELLOW DWARF VIRUS CONTAINS A NOVEL HAMMERHEAD STRUCTURE IN THE SELF-CLEAVAGE DOMAIN
A SATELLITE RNA OF BARLEY YELLOW DWARF VIRUS CONTAINS A NOVEL HAMMERHEAD STRUCTURE IN THE SELF-CLEAVAGE DOMAIN
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DOI：
10.1016/0042-6822(91)91000-7
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发表时间：
1991-08-01
期刊：
影响因子：
3.7
通讯作者：
GERLACH, WL
中科院分区：
文献类型：
--
作者：
MILLER, WA;HERCUS, T;GERLACH, WL
在大麦黄矮病毒(BYDV)RPV血清型分离物中发现了一个具有卫星RNA特性的RNA分子。它有322个核苷酸长，单链，不与病毒基因组杂交。RNA的二聚体，可能代表复制的中间产物，能够自我裂解成单体。在cDNA克隆的试管转录中，能够在正(封装)和负方向上进行自我裂解。在负链裂解位点两侧的序列包含一个一致的“锤头”结构，类似于在其他自裂解卫星RNA中发现的结构。虽然与锤头结构相关，但正链末端两侧的序列显示出与共识的不同，并可能折叠成包含假结的不同结构。
An RNA molecule with properties of a satellite RNA was found in an isolate of barley yellow dwarf virus (BYDV), RPV serotype. It is 322 nucleotides long, single-stranded, and does not hybridize to the viral genome. Dimers of the RNA, which presumably represent replicative intermediates, were able to self-cleave into monomers.In vitrotranscripts from cDNA clones were capable of self-cleavage in both the plus (encapsidated) and minus orientations. The sequence flanking the minus strand cleavage site contained a consensus “ hammerhead” structure, similar to those found in other self-cleaving satellite RNAs. Although related to the hammerhead structure, sequences flanking the plus strand termini showed differences from the consensus and may be folded into a different structure containing a pseudoknot.