Cadherin-mediated cell sorting not determined by binding or adhesion specificity.
Cadherin-mediated cell sorting not determined by binding or adhesion specificity.
复制标题
钙粘蛋白介导的细胞分选不能由结合或粘附特异性确定。
DOI：
10.1083/jcb.200108040
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发表时间：
2002-01-21
影响因子：
7.8
通讯作者：
Gumbiner, Barry M
中科院分区：
文献类型：
--
作者：
Niessen, Carien M;Gumbiner, Barry M
钙粘蛋白粘附分子在组织边界的建立中起重要作用。表达不同钙粘蛋白的细胞在细胞聚集测定中彼此分选。为了确定钙粘蛋白结合和粘附特异性对分选过程的贡献，我们检查了细胞对不同纯化的钙粘蛋白的粘附。中国仓鼠卵巢细胞系表达的四种不同的钙粘蛋白之一，被允许结合到纯化的钙粘蛋白的细胞外结构域的人E-钙粘蛋白或爪蟾C-钙粘蛋白，粘附的特异性进行了比较与细胞分选试验。没有不同的钙粘蛋白表达细胞表现出任何粘附特异性对任何两个纯化的钙粘蛋白基板，即使这些钙粘蛋白在其主要序列有很大的不同。此外，所有细胞在两种基质上均表现出相似的粘附增强。然而，这种缺乏粘附特异性并不能决定不同的钙粘蛋白表达细胞是否会彼此分选，并且分选的趋势不能通过其胞外结构域中序列多样性的程度来预测。这些结果表明，钙粘蛋白的粘附结合能力远比预期的更混杂，并且分选能力必须由简单的粘附结合特异性以外的机制决定。
Cadherin adhesion molecules play important roles in the establishment of tissue boundaries. Cells expressing different cadherins sort out from each other in cell aggregation assays. To determine the contribution of cadherin binding and adhesion specificity to the sorting process, we examined the adhesion of cells to different purified cadherin proteins. Chinese hamster ovary cell lines expressing one of four different cadherins were allowed to bind to the purified cadherin extracellular domains of either human E-cadherin or Xenopus C-cadherin, and the specificity of adhesion was compared with cell-sorting assays. None of the different cadherin-expressing cells exhibited any adhesive specificity toward either of the two purified cadherin substrates, even though these cadherins differ considerably in their primary sequence. In addition, all cells exhibited similar strengthening of adhesion on both substrates. However, this lack of adhesive specificity did not determine whether different cadherin-expressing cells would sort from each other, and the tendency to sort was not predictable by the extent of sequence diversity in their extracellular domains. These results show that cadherins are far more promiscuous in their adhesive-binding capacity than had been expected and that the ability to sort out must be determined by mechanisms other than simple adhesive-binding specificity.