ASSOCIATION OF MITOGEN-ACTIVATED PROTEIN-KINASE WITH THE MICROTUBULE CYTOSKELETON
ASSOCIATION OF MITOGEN-ACTIVATED PROTEIN-KINASE WITH THE MICROTUBULE CYTOSKELETON
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DOI：
10.1073/pnas.92.19.8881
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发表时间：
1995-09-12
影响因子：
11.1
通讯作者：
FISCHER, EH
中科院分区：
文献类型：
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作者：
RESZKA, AA;SEGER, R;FISCHER, EH
使用间接免疫荧光显微镜和生化技术，我们已经确定，在NIH 3T3小鼠成纤维细胞中，大约三分之一的丝裂原活化蛋白激酶（MAPK）与微管细胞骨架有关。这种酶群可以从分布在整个细胞质中的可溶性形式中分离出来，并且在有丝分裂原刺激后也存在于细胞核中。微管相关酶池占有丝分裂刺激后所有可检测到的MARK活性的一半。这些发现扩展了已知的MAPK与微管的体内关联，包括增殖细胞的整个微管细胞骨架，它们表明MARK与微管的直接关联可能是MARK活性与细胞骨架改变之间观察到的相关性的部分原因。
Using indirect immunofluorescence microscopy and biochemical techniques, we have determined that approximately one-third of the total mitogen-activated protein kinase (MAPK) is associated with the microtubule cytoskeleton in NIH 3T3 mouse fibroblasts. This population of enzyme can be separated from the soluble form that is found distributed throughout the cytosol and is also present in the nucleus after mitogen stimulation. The microtubule-associated enzyme pool constitutes half of all detectable MARK activity after mitogenic stimulation. These findings extend the known in vivo associations of MAPK with microtubules to include the entire microtubule cytoskeleton of proliferating cells, and they suggest that a direct association of MARK with microtubules may be in part responsible for the observed correlations between MARK activities and cytoskeletal alteration.