Cytochrome P450-7B1 and 11β-hydroxysteroid dehydrogenase type 1 distribution in human tissues
Cytochrome P450-7B1 and 11β-hydroxysteroid dehydrogenase type 1 distribution in human tissues
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DOI：
10.1515/hmbci-2011-0138
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发表时间：
2012-04-01
影响因子：
1
通讯作者：
Morfin, Robert
中科院分区：
文献类型：
--
作者：
Chalbot, Sonia;Morfin, Robert
细胞色素 P450-7B1 (CYP7B1) 和 1 型 11 β-羟基类固醇脱氢酶 (11 β-HSD1) 对 3 β-羟基类固醇（如 DHEA 和表雄酮）的连续作用导致产生细胞保护性 7 β-羟基化衍生物。可以使用针对 CYP7B1 和 11 beta-HSD1 的免疫组织化学特异性抗体，在市售的人体组织阵列上研究人体组织中是否存在这些酶。这两种酶主要在易于发生炎症的内胚层和外胚层来源的组织中检测到。由于低剂量的 DHEA 和表雄酮的 7 β-羟基化衍生物会触发炎症消退和组织修复，因此 CYP7B1 和 11 β-HSD1 组织含量可能反映病理状况后组织的修复能力。
Successive action of cytochrome P450-7B1 (CYP7B1) and 11 beta-hydroxysteroid dehydrogenase type 1 (11 beta-HSD1) on 3 beta-hydroxysteroids such as DHEA and epiandrosterone leads to the production of cytoprotective 7 beta-hydroxylated derivatives. Investigation of the presence of these enzymes in human tissues could be carried out on commercially available human tissue arrays with use of antibodies specific to CYP7B1 and 11 beta-HSD1 for immunohistochemistry. Both enzymes were detected mainly in tissues of endodermic and ectodermic origin which are prone to undergo inflammation. As low doses of the 7 beta-hydroxylated derivatives of DHEA and epiandrosterone trigger the resolution of inflammation and tissue repair, CYP7B1 and 11 beta-HSD1 tissue contents may reflect the tissue ability for reparation after pathological conditions.