Isolation, Identification, and Molecular Characterization of Strains of Photorhabdus luminescens from Infected Humans in Australia
Isolation, Identification, and Molecular Characterization of Strains of Photorhabdus luminescens from Infected Humans in Australia
复制标题
澳大利亚感染人群中发光杆菌菌株的分离、鉴定和分子表征
DOI：
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发表时间：
1999
影响因子：
9.4
通讯作者：
R. Akhurst
中科院分区：
文献类型：
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作者：
M. Peel;D. Alfredson;J. Gerrard;Jennifer M. Davis;J. Robson;R. McDougall;Barry L. Scullie;R. Akhurst
摘要：我们报道了从4名澳大利亚患者中分离出的发光光habdus(Xenorhabdus): 2名患者有多发性皮肤病变，1名患者只有菌血症，1名患者有弥散性感染。其中一名患者在被蜘蛛咬伤后出现了多处皮肤损伤，而另一名患者的损伤可能与蜘蛛咬伤有关。其余两名患者的感染源尚不清楚。作为肠杆菌科的一员，P. luminescens的不寻常之处在于它不能还原硝酸盐，只发酵葡萄糖和甘露糖。赖氨酸脱羧酶、精氨酸二水解酶和鸟氨酸脱羧酶呈负反应（Moeller）。该物种可移动，利用柠檬酸盐，水解尿素，通常在25°C培养的羊血琼脂板上产生独特类型的环状溶血。微弱的生物发光是其决定性特征。P. luminescens是一种昆虫病原体，与昆虫病原线虫共生。美国以前曾报道从人类临床标本中分离出该病毒。16S rRNA基因的限制性内切片段长度多态性- pcr分析表明，澳大利亚临床菌株与美国临床菌株之间具有高度的相似性，而澳大利亚临床菌株与美国临床菌株之间存在显著差异。然而，对数据的数值分析表明，这两组临床菌株彼此之间的相似性比它们与线虫中发现的共生菌株更相似。这是澳大利亚首次从受感染的人身上分离到发光假单胞菌，也是世界范围内第二次从受感染的人身上分离到该菌。
ABSTRACT We describe the isolation of Photorhabdus(Xenorhabdus) luminescens from four Australian patients: two with multiple skin lesions, one with bacteremia only, and one with disseminated infection. One of the patients had multiple skin lesions following the bite of a spider, while the lesions in the other patient were possibly associated with a spider bite. The source of infection for the remaining two patients is unknown. As a member of the family Enterobacteriaceae, P. luminescens is unusual in that it fails to reduce nitrate and ferments only glucose and mannose. It gives negative reactions for lysine decarboxylase, arginine dihydrolase, and ornithine decarboxylase (Moeller). The species is motile, utilizes citrate, hydrolyzes urea, and usually produces a unique type of annular hemolysis on sheep blood agar plates incubated at 25°C. A weak bioluminescence is the defining characteristic. P. luminescens is an insect pathogen and is symbiotically associated with entomopathogenic nematodes. Its isolation from human clinical specimens has been reported previously from the United States. Restriction fragment length polymorphism-PCR analysis of the 16S rRNA gene demonstrated a high level of similarity among the Australian clinical strains and significant differences between the Australian clinical strains and the U.S. clinical strains. However, numerical analyses of the data suggest that the two groups of clinical strains are more similar to each other than they are to the symbiotic strains found in nematodes. This is the first report of the isolation of P. luminescens from infected humans in Australia and the second report of the isolation of this species from infected humans worldwide.