Detection of a Protein Conformational Equilibrium by Electrospray Ionisation-Ion Mobility-Mass Spectrometry
Detection of a Protein Conformational Equilibrium by Electrospray Ionisation-Ion Mobility-Mass Spectrometry
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DOI：
10.1002/anie.201101077
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发表时间：
2011-01-01
影响因子：
16.6
通讯作者：
Oldham, Neil J.
中科院分区：
文献类型：
--
作者：
Jenner, Matthew;Ellis, Jacqueline;Oldham, Neil J.
符合惯例：通过使用离子淌度光谱法在气相中发现了细胞色素 P450 还原酶的紧凑形式和延伸形式的证据，据信在溶液中以动态平衡存在。两种构象的相对丰度（见图）可能受到电喷雾溶液的离子强度的影响。
Conforming to convention: Evidence for compact and extended forms of cytochrome P450 reductase, believed to exist in dynamic equilibrium in solution, is found in the gas phase by using ion mobility spectrometry. The relative abundance of the two conformations (see picture) can be influenced by the ionic strength of the solution from which they are electrosprayed.