VIBRIO-CHOLERAE PRODUCES A 2ND ENTEROTOXIN, WHICH AFFECTS INTESTINAL TIGHT JUNCTIONS
VIBRIO-CHOLERAE PRODUCES A 2ND ENTEROTOXIN, WHICH AFFECTS INTESTINAL TIGHT JUNCTIONS
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DOI：
10.1073/pnas.88.12.5242
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发表时间：
1991-06-01
影响因子：
11.1
通讯作者：
KAPER, JB
中科院分区：
文献类型：
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作者：
FASANO, A;BAUDRY, B;KAPER, JB
在编码霍乱毒素（CT）的基因中发生特异性突变的减毒霍乱弧菌疫苗株仍然能够引起轻度至中度腹泻。在Ussing室中检查了CT阳性和CT阴性的霍乱弧菌菌株的培养上清液，发现一种毒素通过影响细胞间紧密连接或闭合小带的结构来增加小肠粘膜的通透性。该毒素的活性是可逆的、热不稳定的、对蛋白酶消化敏感的，并且在含有大小在10和30 kDa之间的分子的培养上清液级分中发现。该因子（命名为ZOT，代表闭锁小带毒素）的产生与志愿者中霍乱弧菌菌株的致病性相关，并可能代表感染性腹泻的另一种毒力因子，必须消除该因子才能获得安全有效的霍乱口服活疫苗。
Attenuated Vibrio cholerae vaccine strains specifically mutated in genes encoding cholera toxin (CT) are still capable of causing mild to moderate diarrhea. Culture supernatants of V. cholerae strains, both CT-positive and CT-negative, were examined in Ussing chambers, and a toxin was found that increases the permeability of the small intestinal mucosa by affecting the structure of the intercellular tight junction, or zonula occludens. The activity of this toxin is reversible, heat-labile, sensitive to protease digestion, and found in culture supernatant fractions containing molecules between 10 and 30 kDa in size. Production of this factor (named ZOT for zonula occludens toxin) correlates with diarrheagenicity of V. cholerae strains in volunteers and may represent another virulence factor of infectious diarrhea that must be eliminated to achieve a safe and effective live oral vaccine against cholera.