Transient Transcriptional Regulation of the CYS-C1 Gene and Cyanide Accumulation upon Pathogen Infection in the Plant Immune Response
Transient Transcriptional Regulation of the CYS-C1 Gene and Cyanide Accumulation upon Pathogen Infection in the Plant Immune Response
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DOI：
10.1104/pp.113.219436
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发表时间：
2013-08-01
期刊：
影响因子：
7.4
通讯作者：
Romero, Luis C.
中科院分区：
文献类型：
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作者：
Garcia, Irene;Rosas, Tabata;Romero, Luis C.
氰化物的产生伴随着乙烯的生物合成。拟南芥（Arabidopsis thaliana）解毒氰化物主要通过酶β-氰丙氨酸合酶，主要是由线粒体CYS-C1。CYS-C1功能的丧失对植物没有毒性，并导致CYS-C1突变体中氰化物水平的增加以及根无毛表型。cys-c1和野生型植物中差异表达基因的分类揭示了cys-c1突变体的高内源氰化物含量与生物胁迫反应相关。在亲和和不亲和的植物-细菌互作过程中，氰化物积累与CYS-C1基因表达呈负相关。此外，cys-c1植物呈现出对坏死营养型真菌灰葡萄孢的增加的易感性和对活体营养型假单胞菌番茄致病变种DC 3000细菌和甜菜曲顶病毒的增加的耐受性。cys-c1突变导致叶片呼吸速率降低，活性氧积累，并诱导交替氧化酶AOX 1a和病程相关的PR 1表达。我们假设，氰化物，这是短暂的积累在无毒的细菌感染和组成性积累的cys-c1突变体，解偶联依赖于细胞色素c氧化酶的呼吸电子链，这种解偶联诱导的替代氧化酶的活性和活性氧的积累，这通过刺激水杨酸依赖的信号通路的植物免疫系统的行为。
Cyanide is produced concomitantly with ethylene biosynthesis. Arabidopsis (Arabidopsis thaliana) detoxifies cyanide primarily through the enzyme beta-cyanoalanine synthase, mainly by the mitochondrial CYS-C1. CYS-C1 loss of function is not toxic for the plant and leads to an increased level of cyanide in cys-c1 mutants as well as a root hairless phenotype. The classification of genes differentially expressed in cys-c1 and wild-type plants reveals that the high endogenous cyanide content of the cys-c1 mutant is correlated with the biotic stress response. Cyanide accumulation and CYS-C1 gene expression are negatively correlated during compatible and incompatible plant-bacteria interactions. In addition, cys-c1 plants present an increased susceptibility to the necrotrophic fungus Botrytis cinerea and an increased tolerance to the biotrophic Pseudomonas syringae pv tomato DC3000 bacterium and Beet curly top virus. The cys-c1 mutation produces a reduction in respiration rate in leaves, an accumulation of reactive oxygen species, and an induction of the alternative oxidase AOX1a and pathogenesis-related PR1 expression. We hypothesize that cyanide, which is transiently accumulated during avirulent bacterial infection and constitutively accumulated in the cys-c1 mutant, uncouples the respiratory electron chain dependent on the cytochrome c oxidase, and this uncoupling induces the alternative oxidase activity and the accumulation of reactive oxygen species, which act by stimulating the salicylic acid-dependent signaling pathway of the plant immune system.