ACCUMULATION OF INORGANIC POLYPHOSPHATE IN AEROBACTER AEROGENES .2. ENVIRONMENTAL CONTROL AND ROLE OF SULFUR COMPOUNDS
ACCUMULATION OF INORGANIC POLYPHOSPHATE IN AEROBACTER AEROGENES .2. ENVIRONMENTAL CONTROL AND ROLE OF SULFUR COMPOUNDS
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DOI：
10.1128/jb.86.2.222-231.1963
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发表时间：
1963-01-01
影响因子：
3.2
通讯作者：
SYLVAN, S
中科院分区：
文献类型：
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作者：
HAROLD, FM;SYLVAN, S
无机聚磷酸盐在A.产气菌是生长培养基的函数。在低磷酸盐培养基中，每当核酸合成因营养缺乏而停止时，多磷酸盐就会积累（无论其性质如何）。在高磷培养基中，聚磷酸盐的积累仅由S饥饿诱导。因此，多聚磷酸盐的积累，可以诱导或抑制将通过操纵的S和P含量的培养基。S饥饿的具体要求追溯到存在的细胞内抑制剂聚磷酸盐积累。这是耗尽S饥饿和补充时，硫酸盐恢复。抑制剂被确定为氧化型谷胱甘肽或密切相关的化合物。抑制聚磷酸盐积累需要同时存在高外源性磷酸盐浓度和高细胞内谷胱甘肽水平。由于多磷酸盐合成和降解的稳定状态，抑制多磷酸盐积累导致恒定的多磷酸盐水平。前者以原来一半的速度继续，而后者则急剧加速。能量生成抑制剂可使多磷酸盐代谢的合成和降解阶段完全分离。有人提出，谷胱甘肽加磷酸盐的主要影响是多磷酸盐降解的刺激。多磷酸盐合成似乎是抑制核酸合成的一般结果，但净积累可能被同时降解所掩盖。
The accumulation of inorganic polyphosphate in A. aerogenes was shown to be a function of the growth medium. In low-phosphate medium, polyphosphate accumulated whenever nucleic-acid synthesis ceased due to a nutritional deficiency, (regardless of its nature). In high-phosphate medium polyphosphate accumulation was induced only by S starvation. Polyphosphate accumulation could thus be induced or suppressed at will by manipulation of the S and P content of the medium. The specific requirement for S starvation was traced to the presence of an intracellular inhibitor of polyphosphate accumulation. This was depleted during S starvation and replenished when sulfate was restored. The inhibitor was identified as oxidized glutathione or a closely related compound. Suppression of polyphosphate accumulation required the simultaneous presence of a high exogenous phosphate concentration and a high intracellular glutathione level. Suppression of polyphosphate accumulation resulted in a constant polyphosphate level, due to a steady state of polyphosphate synthesis and degradation. The former continued at half the original rate while the latter was sharply accelerated. The synthetic and degradative phases of polyphosphate metabolism could be completely dissociated by inhibitors of energy generation. It is proposed that the primary effect of glutathione plus phosphate is the stimulation of polyphosphate degradation. Polyphosphate synthesis appears to be a general consequence of the inhibition of nucleic-acid synthesis, but net accumulation may be obscured by concurrent degradation.