A primer set to determine sex in the small Indian mongoose, Herpestes auropunctatus
A primer set to determine sex in the small Indian mongoose, Herpestes auropunctatus
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DOI：
10.1111/j.1755-0998.2010.02931.x
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发表时间：
2011-03-01
影响因子：
7.7
通讯作者：
Kuroiwa, A.
中科院分区：
文献类型：
--
作者：
Murata, C.;Ogura, G.;Kuroiwa, A.
为了使引进的小印度猫鼬，疱疹auropunctatus种群的个体的准确性别鉴定，我们设计了一个引物集的性别特异性片段EIF 2S 3 Y和EIF 2S 3X的扩增。使用该引物组，获得了所有供试基因组DNA样品的预期扩增产物：雄性产生两条带，雌性产生一条带。每个PCR产物的测序证实，从两种性别的DNA样品中扩增的769-bp片段来自EIF 2S 3X，而仅从男性DNA样品中扩增的546-bp片段来自EIF 2S 3 Y。结果表明，该引物组合可用于该物种的性别鉴定。
To enable the accurate sexing of individuals of introduced populations of the small Indian mongoose, Herpestes auropunctatus, we designed a primer set for the amplification of the sex-specific fragments EIF2S3Y and EIF2S3X. Using this primer set, the expected amplification products were obtained for all samples of genomic DNA tested: males yielded two bands and females a single band. Sequencing of each PCR product confirmed that the 769-bp fragment amplified from DNA samples of both sexes was derived from EIF2S3X, whereas the 546-bp fragment amplified only from male DNA samples was derived from EIF2S3Y. The results indicated that this primer set is useful for sex identification in this species.