The zygotic segmentation mutant tailless alters the blastoderm fate map of the Drosophila embryo.
The zygotic segmentation mutant tailless alters the blastoderm fate map of the Drosophila embryo.
复制标题
合子分割突变体无尾改变了果蝇胚胎的胚盘命运图。
DOI：
10.1016/0012-1606(87)90310-1
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发表时间：
1987
影响因子：
2.7
通讯作者：
Lengyel,JA
中科院分区：
文献类型：
--
作者：
Mahoney,PA;Lengyel,JA
无尾（t11）是合子致死突变，其表型在胚胎发生的9小时内观察到，并且包括节段A8、A9和A10的缺失和前叶的减少（Strecker et al.，Dev. 113，64 - 76，1986）。为了确定这种突变的影响是否是由于，如Streckeret等人先前提出的，为了重新编程胚盘命运图，我们将分割基因fushi tarazu（ftz）和hairy（h）的探针与整个胚胎杂交。这些基因的转录本早在第14个核周期就显示出在胚胎中分布的改变，表明该基因在胚盘期起细胞决定作用，并且是theftzandh基因正常表达所必需的。通过测量野生型和野生型胚胎中tzz蛋白条带的位置，我们获得了关于胚盘命运图改变的更精确的信息。从本文和之前报道的结果中，我们得出结论，tll突变导致前外胚叶和后外胚叶位置值的缺失，同时伴随着剩余命运图的扩展。
The well-characterized spatial distributions of the transcripts from severalDrosophilasegmentation genes provide molecular markers which can be used to examine the determination of the segment pattern in early embryos.Tailless (tll)is a zygotic lethal mutation, the phenotype of which is observed by 9 hr of embryogenesis and includes the absence of segments A8, A9, and A10 and a decrease in the procephalic lobe (Strecker et al.,Dev. Biol. 113,64–76,1986). To establish whether this effect of thetllmutation is due, as proposed previously by Streckeret al., to a reprogramming of the blastoderm fate map, we hybridized probes for the segmentation genesfushi tarazu (ftz)andhairy(h) to whole embryos. The transcripts of these genes show an altered distribution intllembryos as early as nuclear cycle 14, indicating that thetllgene acts on cellular determination at the blastoderm stage, and is required for normal expression of theftzandhgenes. We obtained more precise information about the alterations in the blastoderm fate map by measuring the position offtzprotein stripes in wild-type andtllembryos. From the results reported here and previously, we conclude that thetllmutation results in a deletion of anterior and posterior ectodermal positional values, concomitant with an expansion of the remaining fate map.