Transcriptional profiling of the spleen in progressive visceral leishmaniasis reveals mixed expression of type 1 and type 2 cytokine-responsive genes
Transcriptional profiling of the spleen in progressive visceral leishmaniasis reveals mixed expression of type 1 and type 2 cytokine-responsive genes
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DOI：
10.1186/s12865-014-0038-z
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发表时间：
2014-11-26
期刊：
影响因子：
3
通讯作者：
Melby, Peter C.
中科院分区：
文献类型：
--
作者：
Espitia, Claudia M.;Saldarriaga, Omar A.;Melby, Peter C.
背景：叙利亚金仓鼠（Mesocricetus aureus）已被用作研究由许多人类病原体引起的感染的模型。研究仓鼠感染模型的免疫发病机制具有挑战性，因为确定细胞和分子决定因素所需的试剂的可用性有限。结果：我们对仓鼠cDNA文库进行了测序，并开发了第一代自定义cDNA微阵列，其中包括5131个富集免疫应答基因的独特cDNA。我们使用这种微阵列来询问仓鼠脾脏对多诺瓦利什曼原虫的反应，多诺瓦利什曼原虫是一种引起内脏利什曼病的细胞内原生动物。内脏利什曼病的仓鼠模型特别有趣，因为它概括了人类疾病的临床和免疫病理特征，包括恶病质、脾肿大、全血细胞减少、免疫抑制和最终死亡。在微阵列中，差异表达的转录本被鉴定为在未感染组和感染组之间具有至少2倍的表达变化，错误发现率为
Background: The Syrian golden hamster (Mesocricetus aureus) has been used as a model to study infections caused by a number of human pathogens. Studies of immunopathogenesis in hamster infection models are challenging because of the limited availability of reagents needed to define cellular and molecular determinants.Results: We sequenced a hamster cDNA library and developed a first-generation custom cDNA microarray that included 5131 unique cDNAs enriched for immune response genes. We used this microarray to interrogate the hamster spleen response to Leishmania donovani, an intracellular protozoan that causes visceral leishmaniasis. The hamster model of visceral leishmaniasis is of particular interest because it recapitulates clinical and immunopathological features of human disease, including cachexia, massive splenomegaly, pancytopenia, immunosuppression, and ultimately death. In the microarray a differentially expressed transcript was identified as having at least a 2-fold change in expression between uninfected and infected groups and a False Discovery Rate of