Effect of Heat Treatment on the Anticancer Activity of Houttuynia cordata Thunb Aerial Stem Extract in Human Gastric Cancer SGC-7901 Cells
Effect of Heat Treatment on the Anticancer Activity of Houttuynia cordata Thunb Aerial Stem Extract in Human Gastric Cancer SGC-7901 Cells
复制标题
热处理对鱼腥草地上茎提取物对人胃癌SGC-7901细胞抗癌活性的影响
DOI：
10.1080/01635581.2020.1737153
复制
发表时间：
2020-03-16
影响因子：
2.9
通讯作者：
Jiang, Jihong
中科院分区：
文献类型：
--
作者：
Liu, Jinjuan;Zhu, Xinting;Jiang, Jihong
胃癌是世界上最常见的恶性肿瘤之一，通过饮食预防是控制胃癌的方法之一。水葫芦(HCT)是一种具有药食两用功能的植物，具有多种生物学特性。然而，食物风格对HCT抗癌活性的影响尚不清楚。因此，我们研究了热处理对HCT抗癌活性的影响。获得HCT提取物（加热的地上茎、加热的地下茎、加热的叶分别定义为HAS、HSS、HL，未加热的分别定义为NAS、NSS、NL），并通过阿尔玛蓝法检测其抑制活性。DAPI染色及流式细胞仪检测细胞凋亡。Western blot检测凋亡相关蛋白的表达。HCT在4种人肿瘤细胞系中显示出抗肿瘤活性。有趣的是，热处理可以增加抗癌活性。在SCG-7901细胞中，热处理使AS的抗癌活性增加2-14倍，并通过调节内源性信号通路诱导细胞凋亡。有趣的是，caspase 9特异性抑制剂阻断了AS降低的细胞活力。热处理可提高HCT的抗癌活性，可作为预防胃癌的一种饮食方式。
Abstract Gastric cancer is one of the most common malignant tumors in the world, and prevention through diet is one of the ways to control. Houttuynia cordata thunb.(HCT) is a plant having medicine and food function, has many biological properties. However, the effect of food style on the anticancer activity of HCT is not clear. So, we investigate the effect of heat treatment on anticancer activity of HCT. HCT extracts (heated aerial stem, heated subterraneous stem, heated leaves defined as HAS, HSS, HL, respectively, and not heated defined as NAS, NSS, NL, respectively) were obtained, and their inhibited activity were detected by alamar blue assay. The cell apoptosis was detected by DAPI staining and flow cytometry analysis. Western blot was performed to test the expression of apoptotic related protein. HCT showed the anticancer activity in four human tumor cell lines. Interestingly, heat treatment could increase the anticancer activity. In SCG-7901 cells, heat treatment increased anticancer activity of AS by 2-14 folds and induced apoptosis through regulating the intrinsic signaling pathways. Intriguingly, the caspase nine specific inhibitor blocked AS-reduced cell viability. Heat treatment increased the anticancer activity of HCT, and can be used as a dietary style for prevention of gastric cancer.