Effects of Microorganisms on Drop Formation in Microgravity During a Parabolic Flight with Residual Gravity and Jitter
Effects of Microorganisms on Drop Formation in Microgravity During a Parabolic Flight with Residual Gravity and Jitter
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DOI：
10.1007/s12217-022-09933-8
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发表时间：
2022-04-01
影响因子：
1.8
通讯作者：
Hirsa，Amir H.
中科院分区：
文献类型：
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作者：
McMackin，Patrick M.;Adam，Joe A.;Hirsa，Amir H.
湿润和接触线动力学，以及生长（界面形成）和微重力下的微生物液滴的稳定性对于理解和控制空间中的复杂流体非常重要。微重力下的生物液滴研究在生物3D打印、制药生产和生物修复等领域都有应用。这里是微生物的液体培养物。杆菌、S。酿酒酵母（面包用酵母）；在抛物线飞行过程中，使用一根简单的管子将放射性炸弹放置在厘米级的液滴中。抛物线飞行中固有的残余重力和抖动使研究这些力如何影响微重力下生物流体滴的生长成为可能。用无菌培养基与微生物滴液的生长进行了比较。使用准静态模拟来评估每种溶液是否在生长液滴中产生可测量的变化。从液滴长宽比、接触角和重力变化引起的接触角差异等方面分析了生长液滴的图像。结果表明，微生物的存在对厘米级液滴的行为影响很小。微生物对生长中的液滴的影响很小，这预示着现有的地面液滴技术可以适应在失重条件下处理生物流体。
Wetting and contact-line dynamics, as well as growth (interface creation) and stability of aqueous drops with microorganisms in microgravity is important for understanding and controlling complex fluids in space. The study of biofluid drops in microgravity has applications in biological 3D printing, pharmaceutical production, and bioremediation. Here, liquid cultures of the microorganismsE. coli,S. cerevisiae(baker’s yeast), andD. radioduranswere deployed in centimeter-scale drops using a simple tube during a parabolic flight. Residual gravity andg-jitter inherent in parabolic flights allowed for the study of how these forces affect the growth of biofluid drops in microgravity. The growth of drops with microorganisms was compared to sterile growth media. Quasi-static simulations were used to assess whether each solution produced measurable changes in the growing droplet. Images of growing drops were analyzed in terms of drop aspect-ratio, contact angles, and the differences in contact angles due to variations in gravity. Results demonstrate that the presence of microorganisms has minimal influence on the behavior of centimeter-scale drops. The small impact of microorganisms on growing drops bodes well for the adaptation of existing Earth-based drop technologies for working with biofluids in reduced gravity.